LPS-bindendes Protein an humanem Myokard unter Berücksichtigung der parodontalen Gesundheit und des IL-1-Polymorphismus

Semper-Pinnecke, Elske

Eine klinisch-experimentelle Untersuchung bei Patienten mit kardialer Insuffizienz

dc.contributor.advisor	Mausberg, Rainer Prof. Dr.	de
dc.contributor.author	Semper-Pinnecke, Elske	de
dc.date.accessioned	2013-07-31T08:50:55Z	de
dc.date.available	2013-08-27T22:50:03Z
dc.date.issued	2013-07-31	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BAF0-D	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3971
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	LPS-bindendes Protein an humanem Myokard unter Berücksichtigung der parodontalen Gesundheit und des IL-1-Polymorphismus	de
dc.title.alternative	Eine klinisch-experimentelle Untersuchung bei Patienten mit kardialer Insuffizienz	de
dc.type	doctoralThesis	de
dc.title.translated	LPS-binding protein in the human myocardium taking the periodontal health and the IL-1-polymorphism into account	de
dc.contributor.referee	Meyer, Thomas Prof. Dr.	de
dc.date.examination	2013-08-20	de
dc.description.abstracteng	Aim of the present study was to investigate as part of a pilot project whether lipopolysaccharides (LPS) of periodontopathogenic microorganisms are able to ligate to the human myocardium initiating an inflammatory response in the myocardial cells. A quantitative determination of lipopolysaccharide binding protein (LBP) in atrium, ventricle and heart valve was performed and a potential association with the degree of periodontitis severity evaluated. 30 Patients (10 female, 20 male with an average age of 67.7 ±9.3 years) who underwent an aortic valve replacement due to aortic valve stenosis or aortic valve insufficiency were included in the study. After the patient was informed and signed the consent form, a medical history was taken including dental notation (DMFT und Papilla Bleeding Index (PBI)) and a periodontal chart. The degree of periodontitis severity was determined by the probing depth and the attachment level. During surgery, Thoracic and Cardiovascular Surgeons took heart tissue and blood samples. The average DMFT was 21.6 ±4.9. Concerning the PBI, eleven patients had Score 0, eleven patients had Score 1, six patients had Score 2, and two patients had Score 3. 23 patients had a severe periodontitis, five patients a moderate periodontitis, and two atients had no or a mild periodontitis. In the laboratory, the heart tissue samples were analyzed concerning the presence of LBP using protein purification and Western blot analysis. DNA was isolated from the blood samples and tested for IL-1 polymorphism. LBP was found in all patients´ heart tissue samples. The aortic valve displayed the highest big42-LBP expression; the lowest values were observed for the ventricle. eight patients had a genotype 1 (no increased risk for progressive development of periodontitis), eleven had a genotype 2 (increased risk for progressive development of periodontitis), three had a genotype 3 (high risk for progressive development of periodontitis), and eight had a genotype 4 (reduced inhibition of inflammatory reaction). Statistical evaluation showed that there were no significant influences of gender and smoking behavior on the degree of periodontitis severity or the PBI. The IL-1 genotype did also not have an impact on the degree of periodontitis severity (P=0.259) or on the PBI (P=0.167). The quantity of LBP was not associated with the PBI or the IL-1 genotype, but there was an association between LBP in the ventricle and the degree of periodontitis severity (P=0.018). For atrium (P=0.577) and heart valve (P=0.198), no association between LBP and the degree of periodontitis severity was observed. The present results confirm the working hypothesis that LPS of periodontopathogenic microorganisms can ligate to the myocardium and may contribute to heart tissue damage. Further studies are necessary to decipher the responsible mechanisms.	de
dc.contributor.coReferee	Lüder, Carsten Prof. Dr.	de
dc.title.alternativeTranslated	A clinical-experimental examination in patients with cardiac insufficiency	de
dc.subject.ger	Parodontitis	de
dc.subject.ger	Herzinsuffizienz	de
dc.subject.ger	IL-1-Polymorphismus	de
dc.subject.eng	periodontitis	de
dc.subject.eng	cardiac insufficiency	de
dc.subject.eng	IL-1-polymorphism	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BAF0-D-0	de
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.subject.gokfull	Zahn-, Mund- und Kieferheilkunde - Allgemein- und Gesamtdarstellungen (PPN619876360)	de
dc.description.embargoed	2013-08-27	de
dc.identifier.ppn	756328845	de

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Human- und Zahnmedizin [2877]

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