Vergleich der Strahlenwirkung auf Tumorzellkulturen und Tumorstammzellkulturen aus unterschiedlichen Glioblastomen

Oettler, Manuela

dc.contributor.advisor	Virsik-Köpp, Patricia Prof. Dr.
dc.contributor.author	Oettler, Manuela
dc.date.accessioned	2014-04-25T14:17:08Z
dc.date.available	2014-06-06T22:50:04Z
dc.date.issued	2014-04-25
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5E9B-4
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4454
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Vergleich der Strahlenwirkung auf Tumorzellkulturen und Tumorstammzellkulturen aus unterschiedlichen Glioblastomen	de
dc.type	doctoralThesis	de
dc.title.translated	comparison of the effects of radiation on tumor cell cultures and tumor stem cell cultures from different glioblastoma	de
dc.contributor.referee	Stockhammer, Florian PD Dr.
dc.date.examination	2014-05-28
dc.description.abstracteng	In all tests carried out showed a significant resistance of all tumorcells to radiation. Especially in the glioblastoma stem cell cultures, the radiation-induced apoptosis rate was very low. This supports the hypothesis that tumor stem cells are responsible for a new tumor regeneration after successful primary therapy (radiotherapy) in glioblastoma. Furthermore, the formation of multinucleated cells was observed in all cell lines by irradiation. Again, there was a significant difference between glioblastoma multiforme standard cells and stem cells showed. In stem cell cultures, a significantly higher rate polynuclear cells was observed than in the standard cell cultures. These were also in the stem cell cultures over the entire experimental period detectable, where in contrast to the standard cell cultures decreasing with increasing time number was seen to multinuclear cells. Consequently, this indicates a correlation between the formation of multinucleated cells and significantly increased the resistance of stem cells compared to standard cells. It appears that the tumor stem cells through the formation of multinucleated cells have the ability to replace or repair the damaged material, and thus to avoid the cell death. Another part of this work was dedicated with regard to their resistance behavior as a function of oxidative stress the study of glioblastoma standard cell cultures. Natriumselenit as prooxidant led in all three examined standard cell lines to a more rapid induction of apoptosis . The maximum number of apoptotic cells was reached earlier compared to radiotherapy alone by Natriumselenit . The maximum rate of apoptosis induced by 40 µM sodium, was consistent with the induced by irradiation with 7.5 Gy. N-acetylcystein affected, as also known from the literature, the cell lines U251 and U87 antioxidant that is, the apoptosis rate decreased after irradiation with 7.5 Gy. In contrast, the treatment of glioblastoma standard celllinien G112 with N-acetylcystein led to an increase in the apoptosis rate and clarified by a relevant influence of the particular genetic profile of the individual tumor of the connection between oxidative stress and apoptosis in glioblastoma cells.	de
dc.contributor.coReferee	Mausberg, Rainer Prof. Dr.
dc.subject.ger	Resistenz	de
dc.subject.ger	Glioblastomzellen	de
dc.subject.ger	Bestrahlung	de
dc.subject.ger	Glioblastom-Stammzellen	de
dc.subject.ger	strahleninduzierte Apoptoserate	de
dc.subject.ger	Apoptoserate	de
dc.subject.ger	Tumorregenerierung	de
dc.subject.ger	Strahlentherapie	de
dc.subject.ger	mehrkernigen Zellen	de
dc.subject.ger	mehrkernig	de
dc.subject.ger	reparieren	de
dc.subject.ger	Apoptose	de
dc.subject.ger	oxidativer Stress	de
dc.subject.ger	U251	de
dc.subject.ger	G112	de
dc.subject.ger	U87	de
dc.subject.ger	N-Acetylcystein	de
dc.subject.ger	Natriumselenit	de
dc.subject.ger	Glioblastom	de
dc.subject.eng	glioblastoma	de
dc.subject.eng	stem cell cultures	de
dc.subject.eng	tumor	de
dc.subject.eng	radiotherapy	de
dc.subject.eng	regeneration	de
dc.subject.eng	stem cell	de
dc.subject.eng	apoptosis	de
dc.subject.eng	apoptosis rate	de
dc.subject.eng	oxidative stress	de
dc.subject.eng	multinucleated cells	de
dc.subject.eng	U251	de
dc.subject.eng	G112	de
dc.subject.eng	U87	de
dc.subject.eng	N-Acetylcystein	de
dc.subject.eng	Natriumselenit	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5E9B-4-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Neurologie - Allgemein- und Gesamtdarstellungen (PPN619876247)	de
dc.subject.gokfull	Radiologie / Bildgebende Verfahren / Ultraschall / Nuklearmedizin / Strahlenschutz - Allgemein- und Gesamtdarstellungen (PPN619875585)	de
dc.subject.gokfull	GOK-MEDIZIN	de
dc.description.embargoed	2014-06-06
dc.identifier.ppn	783865570

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