Synthesis of fluorescent toxin and nucleotide derivatives to specifically address membrane proteins
von Hanna Agnes Radzey
Datum der mündl. Prüfung:2015-04-01
Erschienen:2015-07-15
Betreuer:Prof. Dr. Ulf Diederichsen
Gutachter:Prof. Dr. Ulf Diederichsen
Gutachter:Prof. Dr. Andreas Janshoff
Gutachter:Prof. Dr. Lutz Ackermann
Gutachter:Prof. Dr. Konrad Koszinowski
Gutachter:Prof. Dr. Claudia Höbartner
Gutachter:Dr. Inke Siewert
Dateien
Name:Hanna Radzey.pdf
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Format:PDF
Zusammenfassung
Englisch
Within this project, new tools for in vivo/in vitro labelling were developed and investigated, which may be utilized in the future to elucidate structures, localisation and distinct processes of biomolecules in their native environment. Fluorescence labelling and detection provides researchers with a highly sensitive, non-destructive method to visualize biomolecules even on the nanometre scale. This thesis comprises two different approaches for fluorescent labelling. The first approach includes the indirect labelling of ion channels, in which ion channels are addressed by specific binding fluorescently labelled neurotoxins. The second project involves a direct labelling approach, where a nucleotide is functionalized with a fluorophore for direct investigation of its concentration and localisation.
Keywords: fluorescent labelling; conotoxin; pompilidotoxin; iberiotoxin; cAMP; disulphide rich peptides; caging