Charakterisierung der Eigenschaften der nichtkodierenden Enden der Genomsegmente des Oropouche-Virus und ihre Bedeutung für die virale Transkription/Replikation sowie die Interaktion mit dem Typ-I-Interferonsystem

Schnülle, Katharina

dc.contributor.advisor	Spiegel, Martin Dr.	de
dc.contributor.author	Schnülle, Katharina	de
dc.date.accessioned	2013-07-25T09:01:14Z	de
dc.date.available	2014-07-08T22:50:05Z
dc.date.issued	2013-07-25	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BADE-C	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3951
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Charakterisierung der Eigenschaften der nichtkodierenden Enden der Genomsegmente des Oropouche-Virus und ihre Bedeutung für die virale Transkription/Replikation sowie die Interaktion mit dem Typ-I-Interferonsystem	de
dc.type	doctoralThesis	de
dc.title.translated	Characterisation of non-coding regions of the OROV genome segments and their relevance for viral transcription/replication as well as interaction with the type-I interferon system	de
dc.contributor.referee	Hufert, Frank Torsten Prof. Dr.	de
dc.date.examination	2013-08-06	de
dc.description.abstracteng	The Oropouche virus (OROV) is a RNA virus from the Bunyaviridae family. Its genome consists of a single-stranded negative sense RNA. The Oropouche fever is caused by this virus and is most common in Central and South America. Moreover it is the second most prevalent arboviral disease after Dengue fever in Brazil and there are no therapeutical options, yet. To guarantee efficient viral replication viruses within this family encode for a non-structural protein (NSs), which operates in an interferon antagonistic function by suppressing the host cell´s RNAP II mediated transcription. Because of this mechanism there is no type-I interferon induction after virus infection. In this thesis I found that through their triphosphate modification at the 5`end the OROV RNA genome segments itself are potent inducers of the interferon system. Moreover type-I interferon is able to sufficiently block OROV replication. Therefore the virus needs an interferon antagonist for efficient replication. The intracellular targets of the NSs protein are still unknown but due to these results their distribution seems to be intranuclear. In addition I established an OROV minireplicon system. Sequence analyses of the viral polymerase und the non-coding ends revealed that the published reference sequences for both are not correct. Especially for the non-coding ends not only the nucleotide sequence itself but the deduced secondary structure seems to be essential for their function.	de
dc.contributor.coReferee	Walter, Lutz Prof. Dr.	de
dc.contributor.thirdReferee	Oppermann, Martin Prof. Dr.	de
dc.subject.ger	Oropouche-Virus	de
dc.subject.ger	Minireplikon-System	de
dc.subject.ger	Bunyaviren	de
dc.subject.eng	Oropouche virus	de
dc.subject.eng	minireplicon system	de
dc.subject.eng	Bunyaviridae	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BADE-C-6	de
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizinische Mikrobiologie / Medizinische Virologie / Medizinische Mykologie / Infektionskrankheiten / Hygiene / Impfung / Parasitologie / Tropenmedizin - Allgemein- und Gesamtdarstellungen (PPN619875356)	de
dc.description.embargoed	2014-07-08	de
dc.identifier.ppn	755811852	de

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