Prädiktive und prognostische Relevanz von p53 und Ki67 beim lokal fortgeschrittenen Rektumkarzinom cUICC-II/III

Posern, Susanna

Immunhistochemische Analysen im methodischen Vergleich

dc.contributor.advisor	Liersch, Torsten PD Dr.
dc.contributor.author	Posern, Susanna
dc.date.accessioned	2013-10-21T09:52:53Z
dc.date.available	2013-11-19T23:50:05Z
dc.date.issued	2013-10-21
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BBF1-3
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4097
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Prädiktive und prognostische Relevanz von p53 und Ki67 beim lokal fortgeschrittenen Rektumkarzinom cUICC-II/III	de
dc.title.alternative	Immunhistochemische Analysen im methodischen Vergleich	de
dc.type	doctoralThesis	de
dc.title.translated	Predictive and prognostic value of p53 and Ki67 in locally advanced rectal cancer cUICC-II/III	de
dc.contributor.referee	Radzun, Heinz-Joachim Prof. Dr.
dc.date.examination	2013-11-12
dc.description.abstracteng	In Germany, colorectal carcinoma represents the second most common cause of death in cancer diseases. The treatment of rectal cancer is stage-dependent and includes a multimodal therapy concept. Currently, the therapeutic regimen for locally advanced rectal cancer involve a neoadjuvant radio-chemotherapy (RCT) followed by the surgical resection of the rectum. Unfortunately approximately 40% of all patients with locally advanced rectal cancer show a poor response to the multimodal therapy. Because of this it is very important to identify an individual risk-profile on the basis of predictive and prognostic biomarkers. This dissertation analyzed the expression of p53 and Ki67 as possible prognostic biomarkers in rectal cancer. An evaluation was done in untreated and residual tumor samples after RCT. The two cellular proteins p53 and Ki67 can be visualized using immunohistochemical staining techniques. The investigated samples derive from 225 patients with locally advanced rectal cancer (cUICC II/III). The obtained immunohistochemical data were correlated with the pathological data (for example TNM classification, tumor regression) and the follow-up-information of the patients. The results of these correlations show a constant pre- and post-therapeutic level of the tumorsupressor p53. Thus p53 expression level seems not be influenced by neoadjuvant RCT. In contrast Ki67 show a significant decreased expression level after neoadjuvant RCT (p<0,05). Probably, these elevated data predict a correlation between the grad of tumor regression and the Ki67 expression. Additionally this dissertation evaluated a digital image analysis system, which was also used for the analyses described previously. Immunohistochemistry is a well-established and widely used method in routine clinical diagnostics as well as in research. Standardization of staining procedures as well as criteria for analyses are still lacking and often requested for this method. Meanwhile, several computer-based evaluation systems are available. All samples were analysed conventionally as well as with the aid of the Ventana Image Analysis System (VIAS). The correlation of both datasets showed a strong correlation of both analysis systems, so the percentage of stained tumor cells, which was captured with both analysis measures showed a nearly linear correlation (correlation coefficient >0,9; p<0.00001).	de
dc.contributor.coReferee	Dobbelstein, Matthias Prof. Dr.
dc.contributor.thirdReferee	Oppermann, Martin Prof. Dr.
dc.title.alternativeTranslated	Immunohistochemical analysis and comparison of analysis methods	de
dc.subject.ger	p53	de
dc.subject.ger	Ki67	de
dc.subject.ger	Rektumkarzinom	de
dc.subject.eng	rectal cancer	de
dc.subject.eng	p53	de
dc.subject.eng	Ki67	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BBF1-3-1
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Abdominalchirurgie (PPN619875976)	de
dc.description.embargoed	2013-11-19
dc.identifier.ppn	770257216

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