Antikanzerogene Effekte von Belamcanda chinensis in Zelllinien maligner Keimzelltumoren des Hodens

Hasibeder, Astrid

dc.contributor.advisor	Schweyer, Stefan PD Dr.
dc.contributor.author	Hasibeder, Astrid
dc.date.accessioned	2013-11-05T11:49:40Z
dc.date.available	2013-11-12T23:50:04Z
dc.date.issued	2013-11-05
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BC23-B
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4129
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4129
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4129
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Antikanzerogene Effekte von Belamcanda chinensis in Zelllinien maligner Keimzelltumoren des Hodens	de
dc.type	doctoralThesis	de
dc.title.translated	Anticancer activities of Belamcanda chinensis in cell lines of malignant testicular germ cell tumors	de
dc.contributor.referee	Schweyer, Stefan PD Dr.
dc.date.examination	2013-11-06
dc.description.abstracteng	Phytoestrogens have been shown to exert anti-proliferative effects on different cancer cells. In addition it could be demonstrated that inhibition of proliferation is associated with downregulation of the known stem cell factors NANOG, POU5F1 and SOX2 in tumor cells. We demonstrate the potential of Belamcanda chinensis extract (BCE) and tectorigenin as anticancer drugs in cell lines of malignant testicular germ cell tumor cells (TGCT) by inhibition of proliferation and regulating the expression of stem cell factors. The TGCT cell lines TCam-2 and NTERA-2 were treated with BCE or tectorigenin and MTT assay was used to measure the proliferation of tumor cells. In addition, the expression of stem cell factors was analyzed by quantitative PCR, western blot analysis and immuncytochemistry. Furthermore, global expression analysis was performed by microarray technique. BCE and tectorigenin inhibited proliferation and downregulated the stem cell factors NANOG and POU5F1 in TGCT cells. In addition, gene expression profiling revealed induction of genes important for the differentiation and inhibition of oncogenes. Hormone receptors known to be effected in prostate cancer cells by BCE or tectorigenin were not regulated in TGCT cells. Utilizing Connectivity Map in an attempt to elucidate mechanism underlying BCE treatments we found highly positive association to histone deacetylase inhibitors (HDACi) amongst others. Causing no histone deacetylase inhibition, the effects of BCE on proliferation and stem cell factors may be based on histone-independent mechanisms such as direct hyperacetylation of transcription factors. Based on these findings, phytoestrogens may be useful as new agents in the treatment of TGCT.	de
dc.contributor.coReferee	Heinrich, Elmar PD Dr.
dc.subject.eng	phytoestrogens	de
dc.subject.eng	testicular germ cell tumors	de
dc.subject.eng	proliferation	de
dc.subject.eng	stem cell factors	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BC23-B-9
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.subject.gokfull	Pathologie {Medizin} (PPN619875682)	de
dc.description.embargoed	2013-11-12
dc.identifier.ppn	770880177

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Human- und Zahnmedizin [2870]

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