Imaging-Analyse dopaminerger Wirkungen am olfaktorischen Nerven von Xenopus-laevis-Larven

Baßfeld, Eiko

dc.contributor.advisor	Schild, Detlev Prof. Dr. Dr.
dc.contributor.author	Baßfeld, Eiko
dc.date.accessioned	2013-11-05T14:12:18Z
dc.date.available	2013-11-13T23:50:04Z
dc.date.issued	2013-11-05
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BC25-7
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4133
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4133
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4133
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Imaging-Analyse dopaminerger Wirkungen am olfaktorischen Nerven von Xenopus-laevis-Larven	de
dc.type	doctoralThesis	de
dc.title.translated	Imaging analysis of dopaminergic effects on the olfactory nerv of xenopus laevis tadpoles.	de
dc.contributor.referee	Schild, Detlev Prof. Dr.
dc.date.examination	2013-11-07
dc.description.abstracteng	Previously it has been demonstrated that dopamine (DA) reduces odor-induced calcium (Ca2+) responses in olfactory receptor neurons in Xenopus laevis tadpoles. This thesis was undertaken to investigate on dopaminergic effects during odor-stimulation in the olfactory nerve (ON). Therefor I established confocal laser imaging of slice preparations of Xenopus laevis tadpoles to record odor-induced Ca2+-response in the ON precisely. Futhermore I found that 15µM-, 25µM-, 50µM-DA reversibly suppresses odor-induced Ca2+-response in the ON. Simultaneously I demonstrated that D2 dopamine receptor (D2R) agonist quinpirole (25µM, 100µM) also reduces Ca2+ transient. The effects of DA was reversed by adding the D2R antagonist sulpiride (100µM, 400µM). It was concluded that the main effects of DA were mediate by D2R. Anymore immunhistochemical dyes has shown tyrosinhydroxylase, a well-established marker on DA metabolism, located in the olfactory ensheating glia cells. Futhermore there was strong evidence that D2R is located at neuronal structures in the ON. These results suggest a neuron-glia interaction within the dopaminergic system in the ON, which maybe point out a process of neuroprotection.	de
dc.contributor.coReferee	Gollisch, Tim Prof. Dr.
dc.contributor.thirdReferee	Virsik-Köpp, Patricia Prof. Dr.
dc.subject.ger	Dopamin	de
dc.subject.ger	Olfaktorik	de
dc.subject.ger	Kalzium-Imaging	de
dc.subject.ger	Xenopus laevis	de
dc.subject.ger	olfaktorischer Nerv	de
dc.subject.ger	Neuroprotektion	de
dc.subject.eng	dopamine	de
dc.subject.eng	olfactory	de
dc.subject.eng	neuroprotection	de
dc.subject.eng	olfactory nerve	de
dc.subject.eng	olfactory system	de
dc.subject.eng	calcium imaging	de
dc.subject.eng	Xenopus laevis	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BC25-7-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Physiologie / Pathophysiologie - Allgemein- und Gesamtdarstellungen (PPN619875283)	de
dc.description.embargoed	2013-11-13
dc.identifier.ppn	770880215

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Human- und Zahnmedizin [2872]

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