dc.contributor.advisor | Thelen, Paul PD Dr. | |
dc.contributor.author | Volland, Alina | |
dc.date.accessioned | 2013-11-07T10:03:21Z | |
dc.date.available | 2013-11-27T23:50:04Z | |
dc.date.issued | 2013-11-07 | |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0001-BC2D-8 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4130 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4130 | |
dc.language.iso | deu | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject.ddc | 610 | de |
dc.title | Molekularpathologie seltener Sarkomentitäten des Urogenitaltraktes | de |
dc.type | doctoralThesis | de |
dc.title.translated | Molecularpathology of rare sarcomas of the genito-urinary tract | de |
dc.contributor.referee | Thelen, Paul PD Dr. | |
dc.date.examination | 2013-11-20 | |
dc.description.abstracteng | Soft tissue sarcomas of the genito-urinary tract are really rare and aggressive tumors with rapid tumor progression and early metastatic disease. Despite the histological similarity of these small-, round- and blue cell tumors, like the Ewing sarcoma family of tumors and rhabdomyosarcomas, there are lots of differences in therapeutic response and prognosis. This shows the importance of a reliable objective molecular marker for quick and exact classification and diagnosing of these tumors.Twenty paraffin-embedded, formalin-fixated tumor tissues and one fresh frozen tumor sample of the entities of the Ewing sarcoma family of tumors and embryonal rhabdomyosarcoma were analysed via qRT-PCR for the expression of the specific fusiontranscripts EWS/FLI1 type 1 and 2, EWS/ERG, PAX3 and PAX7/FKHR. There was a specific expression of the analyzed fusiontranscripts with one positive paraffin-embedded, formalin-fixated tumor tissue sample for the Ewing sarcoma family of tumors-specific translocation EWS/FLI1 type 2 and a positive expression of the same fusiontranscript in the fresh frozen tumor sample.Because of the very poor RNA quality it was necessary to produce a positive standard for one of the fusiontranscripts EWS/FLI1 type 2. All paraffin-embedded, formalin-fixated samples showed a highly degraded RNA with a RIN of 1,0 till 2,5. This shows that molecular analyzes are possible in paraffin-embedded, formalin-fixated tumor tissues, but nevertheless often they are limited to the poor RNA quality.Alternatives in tissue fixation and storage may help to achieve a better RNA quality for molecular analyzes of these fusiontranscripts, so that they can become a standard examination in the clinical daily life for exact and quick diagnosing of these rare and aggressive tumors. | de |
dc.contributor.coReferee | Ströbel, Philipp Prof. Dr. | |
dc.subject.ger | Weichteilsarkom | de |
dc.subject.ger | Ewing Tumor | de |
dc.subject.ger | Rhabdomyosarkom | de |
dc.subject.ger | EWS/FLI1 | de |
dc.subject.ger | RNA Qualität | de |
dc.subject.eng | soft tissue sarcomas | de |
dc.subject.eng | Ewing sarcoma family of tumors | de |
dc.subject.eng | rhabdomyosarcoma | de |
dc.subject.eng | EWS/FLI1 | de |
dc.subject.eng | RNA quality | de |
dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BC2D-8-8 | |
dc.affiliation.institute | Medizinische Fakultät | de |
dc.subject.gokfull | Urologie / Andrologie - Allgemein- und Gesamtdarstellungen (PPN619876131) | de |
dc.description.embargoed | 2013-11-27 | |
dc.identifier.ppn | 770990614 | |