Molekularpathologie seltener Sarkomentitäten des Urogenitaltraktes

Volland, Alina

dc.contributor.advisor	Thelen, Paul PD Dr.
dc.contributor.author	Volland, Alina
dc.date.accessioned	2013-11-07T10:03:21Z
dc.date.available	2013-11-27T23:50:04Z
dc.date.issued	2013-11-07
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BC2D-8
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4130
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4130
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Molekularpathologie seltener Sarkomentitäten des Urogenitaltraktes	de
dc.type	doctoralThesis	de
dc.title.translated	Molecularpathology of rare sarcomas of the genito-urinary tract	de
dc.contributor.referee	Thelen, Paul PD Dr.
dc.date.examination	2013-11-20
dc.description.abstracteng	Soft tissue sarcomas of the genito-urinary tract are really rare and aggressive tumors with rapid tumor progression and early metastatic disease. Despite the histological similarity of these small-, round- and blue cell tumors, like the Ewing sarcoma family of tumors and rhabdomyosarcomas, there are lots of differences in therapeutic response and prognosis. This shows the importance of a reliable objective molecular marker for quick and exact classification and diagnosing of these tumors.Twenty paraffin-embedded, formalin-fixated tumor tissues and one fresh frozen tumor sample of the entities of the Ewing sarcoma family of tumors and embryonal rhabdomyosarcoma were analysed via qRT-PCR for the expression of the specific fusiontranscripts EWS/FLI1 type 1 and 2, EWS/ERG, PAX3 and PAX7/FKHR. There was a specific expression of the analyzed fusiontranscripts with one positive paraffin-embedded, formalin-fixated tumor tissue sample for the Ewing sarcoma family of tumors-specific translocation EWS/FLI1 type 2 and a positive expression of the same fusiontranscript in the fresh frozen tumor sample.Because of the very poor RNA quality it was necessary to produce a positive standard for one of the fusiontranscripts EWS/FLI1 type 2. All paraffin-embedded, formalin-fixated samples showed a highly degraded RNA with a RIN of 1,0 till 2,5. This shows that molecular analyzes are possible in paraffin-embedded, formalin-fixated tumor tissues, but nevertheless often they are limited to the poor RNA quality.Alternatives in tissue fixation and storage may help to achieve a better RNA quality for molecular analyzes of these fusiontranscripts, so that they can become a standard examination in the clinical daily life for exact and quick diagnosing of these rare and aggressive tumors.	de
dc.contributor.coReferee	Ströbel, Philipp Prof. Dr.
dc.subject.ger	Weichteilsarkom	de
dc.subject.ger	Ewing Tumor	de
dc.subject.ger	Rhabdomyosarkom	de
dc.subject.ger	EWS/FLI1	de
dc.subject.ger	RNA Qualität	de
dc.subject.eng	soft tissue sarcomas	de
dc.subject.eng	Ewing sarcoma family of tumors	de
dc.subject.eng	rhabdomyosarcoma	de
dc.subject.eng	EWS/FLI1	de
dc.subject.eng	RNA quality	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BC2D-8-8
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Urologie / Andrologie - Allgemein- und Gesamtdarstellungen (PPN619876131)	de
dc.description.embargoed	2013-11-27
dc.identifier.ppn	770990614

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