Monitoring von Membranen und membrangebundenen Dehydrogenasen in Essigsäurebakterien

Kokoschka, Sebastian

dc.contributor.advisor	Hoppert, Michael PD Dr.
dc.contributor.author	Kokoschka, Sebastian
dc.date.accessioned	2013-11-20T10:10:25Z
dc.date.available	2013-11-20T10:10:25Z
dc.date.issued	2013-11-20
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0001-BC6E-7
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4176
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	570	de
dc.title	Monitoring von Membranen und membrangebundenen Dehydrogenasen in Essigsäurebakterien	de
dc.type	doctoralThesis	de
dc.title.translated	Monitoring of membranes and membrane-bound dehydrogenases in acetic acid bacteria	de
dc.contributor.referee	Hoppert, Michael PD Dr.
dc.date.examination	2013-10-21
dc.description.abstracteng	The occurrence of intracytoplasmic membranes (ICM) in Gluconobacter oxydans was first described by Claus et al. in 1975. The presence of the ICM was observed during the early stationary growth phase. It was proposed, that an increased turnover of the used substrate glycerol at the same time is related to the presence of the ICM. Concerning the possible use of the effect for the biotechnological application, these issues are examined in this dissertation by using Western-Blot analysis, the immunogold-labeling technique for the localization of membrane-bound enzymes via transmission electron microscopy (TEM) and the determination of the specific activity of membrane bound, pyrroloquinolin quinine (PQQ) dependent dehydrogenases. Overall, the obtained data indicates that there is no clear connection between the occurrence of the ICM and the specific activity of membrane bound, PQQ dependent dehydrogenases and therefore it seems unlikely that the ICM could be useful in the improvement of the biotechnological application of Gluconobacter oxydans. In the end however, further investigations are proposed to obtain additional insides into the ICM and the possibility of using them as a platform for membrane-bound dehydrogenases in Gluconobacter oxydans.	de
dc.contributor.coReferee	Stülke, Jörg Prof. Dr.
dc.subject.ger	Mikrobiologie	de
dc.subject.ger	Gluconobacter oxydans	de
dc.subject.ger	Intracytoplasmatische Membranen	de
dc.subject.ger	Membrangebundene Dehydrogenasen	de
dc.subject.ger	Transmissionselektronenmikroskopie	de
dc.subject.ger	Immunogoldmarkierung	de
dc.subject.eng	Microbiology	de
dc.subject.eng	Gluconobacter oxydans	de
dc.subject.eng	Intracytoplasmic membranes	de
dc.subject.eng	Membrane-bound dehydrogenases	de
dc.subject.eng	Transmission electron microscopy	de
dc.subject.eng	Immunogold-labeling	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0001-BC6E-7-7
dc.affiliation.institute	Biologische Fakultät für Biologie und Psychologie	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	772017433

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