Theoretical and experimental study of protein-lipid interactions

Ivanova, Vesselka Petrova

dc.contributor.advisor	Heimburg, Thomas PD Dr.	de
dc.contributor.author	Ivanova, Vesselka Petrova	de
dc.date.accessioned	2000-12-13T15:34:38Z	de
dc.date.accessioned	2013-01-18T13:34:34Z	de
dc.date.available	2013-01-30T23:51:08Z	de
dc.date.issued	2000-12-13	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0006-B5A6-8	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-2738
dc.format.mimetype	ContentType:application/pdf Size:21648	de
dc.language.iso	eng	de
dc.rights.uri	http://webdoc.sub.gwdg.de/diss/copyrdiss.htm	de
dc.title	Theoretical and experimental study of protein-lipid interactions	de
dc.type	doctoralThesis	de
dc.title.translated	Theoretische und experimentelle Untersuchung von Protein-Lipid Wechselwirkungen	de
dc.contributor.referee	Kree, Reiner Prof. Dr.	de
dc.date.examination	2000-11-01	de
dc.subject.dnb	530 Physik	de
dc.description.abstracteng	The thermotropic behavior of a lipid membrane incorporating small integral proteins is investigated. The theory is based on the two-state Ising model of which the distribution of states is explored by Monte Carlo simulations. The complete information of the degeneracy of states of a single-component lipid monolayer system is sampled in one broad histogram. The histogram method for Monte Carlo data analysis is first applied to generate fits of experimentally measured heat capacity profiles of three different vesicle preparations. In the general case of a lipid membrane incorporating small peptides, two component Monte Carlo simulations are performed. The peptide aggregation within the membrane plane results from the different solubility in the gel and the fluid phase. The information about the density of states in this case is sampled in a broad four-dimensional histogram. From the comparison of the theoretical calculations to the experimentally measured heat capacity profiles, the aggregation of the peptide gramicidin A in the gel and the fluid phase of DPPC and DMPC bilayer is predicted. These predictions are tested by means of the atomic force microscopy technique and a good agreement is found. The information sampled in the monolayer histogram is used to resolve lipid relaxation properties near the chain-melting transition. The relaxation times resolved characterize the decay of the enthalpy fluctuations. The proportionality relation between the relaxation time and the heat capacity, estimated theoretically agrees well with the results by pressure-calorimetry experiments.	de
dc.contributor.coReferee	Heimburg, Thomas PD Dr.	de
dc.subject.topic	Mathematics and Computer Science	de
dc.subject.eng	lipid membranes	de
dc.subject.eng	peptides	de
dc.subject.eng	Monte Carlo	de
dc.subject.eng	histogram method	de
dc.subject.eng	calorimetry	de
dc.subject.eng	AFM	de
dc.subject.eng	lipid kinetics	de
dc.subject.bk	33.25	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-894-5	de
dc.identifier.purl	webdoc-894	de
dc.affiliation.institute	Fakultät für Physik	de
dc.subject.gokfull	RJT 000: Anwendungen der Thermodynamik	de
dc.identifier.ppn	326787666

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