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dc.contributor.advisor Heimburg, Thomas PD Dr. de
dc.contributor.author Ivanova, Vesselka Petrova de
dc.date.accessioned 2000-12-13T15:34:38Z de
dc.date.accessioned 2013-01-18T13:34:34Z de
dc.date.available 2013-01-30T23:51:08Z de
dc.date.issued 2000-12-13 de
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-0006-B5A6-8 de
dc.format.mimetype ContentType:application/pdf Size:21648 de
dc.language.iso eng de
dc.rights.uri http://webdoc.sub.gwdg.de/diss/copyrdiss.htm de
dc.title Theoretical and experimental study of protein-lipid interactions de
dc.type doctoralThesis de
dc.title.translated Theoretische und experimentelle Untersuchung von Protein-Lipid Wechselwirkungen de
dc.contributor.referee Kree, Reiner Prof. Dr. de
dc.date.examination 2000-11-01 de
dc.subject.dnb 530 Physik de
dc.description.abstracteng The thermotropic behavior of a lipid membrane incorporating small integral proteins is investigated. The theory is based on the two-state Ising model of which the distribution of states is explored by Monte Carlo simulations. The complete information of the degeneracy of states of a single-component lipid monolayer system is sampled in one broad histogram. The histogram method for Monte Carlo data analysis is first applied to generate fits of experimentally measured heat capacity profiles of three different vesicle preparations. In the general case of a lipid membrane incorporating small peptides, two component Monte Carlo simulations are performed. The peptide aggregation within the membrane plane results from the different solubility in the gel and the fluid phase. The information about the density of states in this case is sampled in a broad four-dimensional histogram. From the comparison of the theoretical calculations to the experimentally measured heat capacity profiles, the aggregation of the peptide gramicidin A in the gel and the fluid phase of DPPC and DMPC bilayer is predicted. These predictions are tested by means of the atomic force microscopy technique and a good agreement is found. The information sampled in the monolayer histogram is used to resolve lipid relaxation properties near the chain-melting transition. The relaxation times resolved characterize the decay of the enthalpy fluctuations. The proportionality relation between the relaxation time and the heat capacity, estimated theoretically agrees well with the results by pressure-calorimetry experiments. de
dc.contributor.coReferee Heimburg, Thomas PD Dr. de
dc.subject.topic Mathematics and Computer Science de
dc.subject.eng lipid membranes de
dc.subject.eng peptides de
dc.subject.eng Monte Carlo de
dc.subject.eng histogram method de
dc.subject.eng calorimetry de
dc.subject.eng AFM de
dc.subject.eng lipid kinetics de
dc.subject.bk 33.25 de
dc.identifier.urn urn:nbn:de:gbv:7-webdoc-894-5 de
dc.identifier.purl webdoc-894 de
dc.affiliation.institute Fakultät für Physik de
dc.subject.gokfull RJT 000: Anwendungen der Thermodynamik de
dc.identifier.ppn 326787666

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