dc.contributor.advisor | Matthaei, Johannes Heinrich Prof. Dr. | de |
dc.contributor.author | Mesa Herrera, Natalia Regina | de |
dc.date.accessioned | 2012-05-16T12:09:14Z | de |
dc.date.available | 2013-01-30T23:50:40Z | de |
dc.date.issued | 2007-08-02 | de |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0006-B627-B | de |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-1309 | |
dc.description.abstract | Der Weissbüschelaffe Callithrix jacchus dient
als Tiermodell zur Erforschung von Infektionen,
Arzneimittelwirksamkeiten, der Parkinson-Krankheit
sowie Organtransplantationen. Ferner wurde an ihm die
Durchführbarkeit von Zell- und Gentherapie bei
immunologischen und hämatologischen Störungen getestet.
Dieser Neuweltaffe ist von besonderer Bedeutung wegen
seiner Ähnlichkeit zum Menschen. Ziel dieser Arbeit war
es die vier Klasse-I-Intervalle (Contig 1-4),
einschließlich der flankierenden Framework-Gene, der
MHC-Klasse-I-Region des Weissbüschelaffen Callithrix
jacchus auf genomischer Ebene zu analysieren.
Hierzu wurde eine BAC-Bank (CHORI 259) von
Callithrix jacchus mit Sonden von zwei
MHC-Klasse-I-Genen und mehreren Framework-Genen
gescreent. Southern-Blot- und Sequenzanalyse der
MHC-Klasse-I-positiven BAC-Klone ermöglichte die
Erstellung einer Karte über die Anordnung der
MHC-Klasse-I-Gene im Weissbüschelaffen. Die
physikalische Kartierung der MHC-Klasse-I-Region von
Callithrix jacchus liefert neue Grundlagen zur
weiteren Analyse der MHC Evolution und zur Erforschung
von verschiedenen Krankheiten, die mit MHC assoziert
sind. Außerdem dient die Karte als Grundlage für die
komplette Sequenzierung der MHC Region. | de |
dc.format.mimetype | application/pdf | de |
dc.language.iso | eng | de |
dc.rights.uri | http://webdoc.sub.gwdg.de/diss/copyr_diss.html | de |
dc.title | Pioneering studies on the gene order, DNA sequence and evolution of the MHC class I region in the new world primate Callthrix jacchus | de |
dc.type | doctoralThesis | de |
dc.title.translated | Pionieruntersuchungen der Gen-Reihenfolge, DNA-Sequenz und Evolution der MHC-Klasse-I-Region in Neue-Welt-Primaten Callithrix jacchus | de |
dc.contributor.referee | Wienands, Jürgen Prof. Dr. | de |
dc.date.examination | 2007-07-05 | de |
dc.subject.dnb | 500 Naturwissenschaften allgemein | de |
dc.description.abstracteng | Analysis of MHC class I region in common marmoset
(Callithrix jacchus) was made with 42 BAC
clones. These clones containing class I region genes
were identified for the purpose of both restriction
mapping and PCR sequencing. Contigs 1, 3 and 4 of BAC
clones were obtained for genomic analysis by comparison
of EcoRI fragments characterized by Southern blot
fingerprint hybridization with framework and class I
gene probes. A gene map for the interval BAT1 - PUO5F1
was constructed by comparison of the Caja EcoRI
fragments so characterized with the computer-split
EcoRI fragments of the Hs class I region having known
gene assignments. Ancillary evidence needed for
identification of a fragment obtained from six BAC
clones was obtained by analysis of sections of the Hs
DNA sequence near POU5F1 for class I pseudogenes using
a collection of all Hs class I pseudogene sequences for
comparison of their alignment by the neighbour-joining
program. Seven new pseudogenes were thereby detected
within only 50 kb from POU5F1, each one classified by
its greatest similarity with a known pseudogene.
Adequately refined, this method might be useful for
finding and classifying all of the many class I
pseudogenes. A genetic typification of 42 BAC clone
inserts by PCR with various primers was performed. This
knowledge was used for pilot sequencing in the class I
region of Caja. The resulting 55 sequences were
localized and potentially gene-assigned by comparative
alignment, generally with DNA of Hs, in many cases also
of other primates and mammals. These sequence
localizations and assignments were defined by their
first and last bp numbers on the scales of Shiina and
also of the Venter genome. For survey, they were both
tabulated and entered into a to-bp-scale map drawn for
the Hs class I region. TS1, one of the two Caja
sequences established by Prof. Takashi Shiina using a
BAC clone selected by the author, was shown to contain
within its 2994 bp four different pseudogenes of
peculiar interest: on the right hand strand, two class
I pseudogenes (ps1 and ps2); on the left hand strand,
reading anti-parallel, a rather young ferritine heavy
chain pseudogene (psfth1) apparently unique to Caja;
and next to it exist a Vorspann v, potentially an
ancient 5 FR (flanking region) which in its
reverse-complementary, the right hand strand turns out
to be the known pseudogene HCGIV-02, as a very highly
identical, almost completely coincident sequence. The
two new pseudogenes, ps1 and ps2, were recognized as
being useful indicators for the duplicated DNA as found
in the two duplicons B and C of Hs and Patr that
contain the ps1, ps2 and v homologues near the
classical HLA genes B and C, respectively. Alignment of
ps1, ps2 and v could be observed as well in all of the
14 Mamu B duplicons of the rhesus monkey Macacca
mulatta. High identity alignment of ps1, ps2 and v
is being searched for in the vicinities of other
classical HLA genes being conditions for duplicons.
Physical mapping and sequence analysis of the MHC class
I region of Callithrix jacchus is to supply new
genomic basic data, which further analyses of the MHC
region for evolution of MHC, disease susceptibility,
immune reactivity and transplantation repulsion of the
MHC to make possible. Additionally the map offers the
basis for following Sequencing of the BAC clones of the
individual contigs. | de |
dc.contributor.coReferee | Wiemmer, Ernts A. Prof. Dr. | de |
dc.subject.topic | Mathematics and Computer Science | de |
dc.subject.ger | MHC | de |
dc.subject.ger | Klasse-I-Region | de |
dc.subject.ger | Callithrix jacchus | de |
dc.subject.ger | Framework-Gen | de |
dc.subject.ger | Evolution | de |
dc.subject.eng | MHC | de |
dc.subject.eng | class I region | de |
dc.subject.eng | Callithrix jacchus | de |
dc.subject.eng | framework gene | de |
dc.subject.eng | evolution | de |
dc.subject.bk | 42.64 | de |
dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-1544-0 | de |
dc.identifier.purl | webdoc-1544 | de |
dc.affiliation.institute | Medizinische Fakultät | de |
dc.subject.gokfull | WJL 000: Molekulargenetik {Biologie} | de |
dc.identifier.ppn | 584435029 | de |