Isolation and characterization of tissue and development-specific microRNAs from Drosophila, mouse and human

Lagos-Quintana, Mariana

dc.contributor.advisor	Tuschl, Thomas Dr.	de
dc.contributor.author	Lagos-Quintana, Mariana	de
dc.date.accessioned	2012-05-16T12:12:56Z	de
dc.date.available	2013-01-30T23:50:31Z	de
dc.date.issued	2005-07-19	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0006-B6E7-C	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-1422
dc.format.mimetype	application/pdf	de
dc.language.iso	eng	de
dc.rights.uri	http://webdoc.sub.gwdg.de/diss/copyr_diss.html	de
dc.title	Isolation and characterization of tissue and development-specific microRNAs from Drosophila, mouse and human	de
dc.type	doctoralThesis	de
dc.title.translated	Isolierung und Charakterisierung von gewebs- und entwicklungs-spezifischen mikroRNAs von Drosophila, Maus und Mensch	de
dc.contributor.referee	Tuschl, Thomas Dr.	de
dc.date.examination	2005-05-02	de
dc.subject.dnb	500 Naturwissenschaften allgemein	de
dc.description.abstracteng	MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression in plants, animals and viruses. These small RNAs provide specificity to protein effector complexes mediating posttranscriptional silencing. The Caenorhabditis elegans lin-4 gene was the first miRNA characterized and for a long time was deemed to be unique to nematodes. Later, the let-7 miRNA was discovered in C. elegans, and it was found to be evolutionary conserved in the genomes of Drosophila melanogaster and human. This work describes the cloning of small RNAs from Drosophila, mouse and human. Among the cloned RNAs was let-7 and many novel invertebrate and mammalian miRNAs.Several miRNAs were found to be tissue-specific and developmentally regulated. Besides miRNAs, repeat-associated small interfering RNAs were cloned from Drosophila; this class of small regulatory RNAs derived from transposons and other repeat regions from fly. During the last few years miRNAs have been further characterized by many laboratories. Their mode of expression and biosynthesis pathway have been studied in depth, and proteins involved in the miRNA-guided silencing pathway have been identified. The current understanding of miRNA and related RNA silencing phenomena is presented. In addition, this work describes the optimization and validation of the cloning procedure used for the discovery of miRNAs. The improvements of the protocol are discussed in the context of other small RNA profiling methods.	de
dc.contributor.coReferee	Lührmann, Reinhard Prof. Dr.	de
dc.subject.topic	Mathematics and Computer Science	de
dc.subject.eng	development-specific microRNAs	de
dc.subject.eng	entwicklungs-spezifischen mikroRNAs	de
dc.subject.bk	30	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-86-4	de
dc.identifier.purl	webdoc-86	de
dc.affiliation.institute	Biologische Fakultät inkl. Psychologie	de
dc.subject.gokfull	RA	de
dc.identifier.ppn	50268433X	de

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Fakultät für Biologie und Psychologie (inkl. GAUSS) [1621]

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