Requirement of HSP70s in the cytosol to vacuole transport of aminopeptidase 1 in Saccharomyces cerevisiae

by Chitkala Satyanarayana
Doctoral thesis
Date of Examination:2000-11-01
Date of issue:2000-10-27
Advisor:Prof. Dr. Dr. h.c. Kurt von Figura
Referee:Prof. Dr. Dr. h.c. Kurt von Figura
Referee:Prof. Dr. David Gordon Robinson
Persistent Address: http://dx.doi.org/10.53846/goediss-1424

 

 

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Abstract

English

In Saccharomyces cerevisiae, aminopeptidase 1 (Ape1p) is a vacuolar resident protein which is transported to the vacuole by a non classical pathway. On synthesis in the cytoplasm the protein assembles into a homododecamer and is transported directly into the vacuole. It does not take the classical secretory pathway which is taken by most of the vacuolar resident proteins. A translocation as well as a vesicle mediated process have been suggested to describe the cytosol to vacuole targeting of Ape1p. Heat shock proteins of the 70 kDa family are known to assist in the transport of proteins into various organelles like the mitochondria, endoplasmic reticulum and nucleus. In this project we determined that the cytosolic members of the heat shock proteins of the 70 kDa family (HSP70s) assist in the transport of aminopeptidase 1 to the vacuole.
Keywords: Cytosol to vacuole transport; aminopeptidase 1; heat shock proteins
 
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