dc.contributor.advisor | Brenig, Bertram Prof. Dr. Dr. | de |
dc.contributor.author | Spittau, Gabriele | de |
dc.date.accessioned | 2012-11-15T15:10:20Z | de |
dc.date.accessioned | 2013-01-18T10:19:10Z | de |
dc.date.available | 2013-01-30T23:51:20Z | de |
dc.date.issued | 2012-11-15 | de |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-EF49-C | de |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-1948 | |
dc.format.mimetype | application/pdf | de |
dc.language.iso | ger | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
dc.title | Untersuchungen zur Rolle von Klf10 und Klf11 als Mediatoren von NGF- und TGF-β-vermittelten Effekten in Zellen neuraler Herkunft | de |
dc.type | doctoralThesis | de |
dc.title.translated | Investigations into the role of Klf10 and Klf11 as mediators of NGF- and TGF-beta-mediated effects in cells of neural origin | de |
dc.contributor.referee | Brenig, Bertram Prof. Dr. Dr. | de |
dc.date.examination | 2011-11-17 | de |
dc.subject.dnb | 000 Allgemeines | de |
dc.subject.dnb | Wissenschaft | de |
dc.subject.gok | WKF 300 | de |
dc.description.abstracteng | The TGF-β1/Klf-family of transcription
factors. They play important roles in development and maintenance
of various tissues and cell types.
The publications consolidated in this thesis describe, that Klf11
mimics TGF-β effects by inducing apoptotsis accompanied by
activation of cysteine-dependent aspartate-specific protease 3
(Caspase-3). Further the thesis demonstrates that Klf11 represses
the expression of the inhibitory Sma and Mad related protein 7
(Smad7) and disrupts the negative-feedback loop of the TGF-β
signaling pathway. Loss of the N-terminal repression domains of
Klf11 abrogates its proapoptotic nature and the repression of
Smad7. In conclusion, the evidence was given that the transcription
factor Klf11 is a downstream mediator of TGF-β-induced apoptosis
and a potent modulator of the TGF-β signaling pathway in the
oligodendroglial cell line Oli-neu.
In a second part of this thesis it has been shown by using
semiquantitative PCR and immunoblotting, that nerve growth factor
(NGF), a member of the neurotrophin family, regulates the
expression of Klf10 in the pheochromocytoma cell line PC12 in a
tropomyosin related kinase A- (TrkA) dependent manner. Moreover,
the thesis provides evidence for the existence of NGF-responsive
elements in the 5 | de |
dc.contributor.coReferee | Knorr, Christoph Prof. Dr. | de |
dc.contributor.thirdReferee | Krieglstein, Kerstin Prof. Dr. | de |
dc.subject.topic | Agricultural Sciences | de |
dc.subject.ger | Klf10 | de |
dc.subject.ger | Klf11 | de |
dc.subject.ger | Sp1-/Krüppelähnliche Transkriptionsfaktoren | de |
dc.subject.ger | Apoptose | de |
dc.subject.ger | Zellzyklusarrest | de |
dc.subject.ger | TGF-β | de |
dc.subject.ger | NGF | de |
dc.subject.ger | Oli-neu-Zellen | de |
dc.subject.ger | PC12-Zellen | de |
dc.subject.eng | Klf10 | de |
dc.subject.eng | KLF11 | de |
dc.subject.eng | Sp1-/Krüppelähnliche transcription factors | de |
dc.subject.eng | apoptosis | de |
dc.subject.eng | cell cycle arrest | de |
dc.subject.eng | TGF-β | de |
dc.subject.eng | NGF | de |
dc.subject.eng | Oli-neu-cells | de |
dc.subject.eng | PC12-cells | de |
dc.subject.bk | ??? | de |
dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-3793-8 | de |
dc.identifier.purl | webdoc-3793 | de |
dc.affiliation.institute | Fakultät für Agrarwissenschaften | de |
dc.identifier.ppn | 733998909 | de |