Untersuchungen zur Rolle von Klf10 und Klf11 als Mediatoren von NGF- und TGF-β-vermittelten Effekten in Zellen neuraler Herkunft

Spittau, Gabriele

dc.contributor.advisor	Brenig, Bertram Prof. Dr. Dr.	de
dc.contributor.author	Spittau, Gabriele	de
dc.date.accessioned	2012-11-15T15:10:20Z	de
dc.date.accessioned	2013-01-18T10:19:10Z	de
dc.date.available	2013-01-30T23:51:20Z	de
dc.date.issued	2012-11-15	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-EF49-C	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-1948
dc.format.mimetype	application/pdf	de
dc.language.iso	ger	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	Untersuchungen zur Rolle von Klf10 und Klf11 als Mediatoren von NGF- und TGF-β-vermittelten Effekten in Zellen neuraler Herkunft	de
dc.type	doctoralThesis	de
dc.title.translated	Investigations into the role of Klf10 and Klf11 as mediators of NGF- and TGF-beta-mediated effects in cells of neural origin	de
dc.contributor.referee	Brenig, Bertram Prof. Dr. Dr.	de
dc.date.examination	2011-11-17	de
dc.subject.dnb	000 Allgemeines	de
dc.subject.dnb	Wissenschaft	de
dc.subject.gok	WKF 300	de
dc.description.abstracteng	The TGF-β1/Klf-family of transcription factors. They play important roles in development and maintenance of various tissues and cell types. The publications consolidated in this thesis describe, that Klf11 mimics TGF-β effects by inducing apoptotsis accompanied by activation of cysteine-dependent aspartate-specific protease 3 (Caspase-3). Further the thesis demonstrates that Klf11 represses the expression of the inhibitory Sma and Mad related protein 7 (Smad7) and disrupts the negative-feedback loop of the TGF-β signaling pathway. Loss of the N-terminal repression domains of Klf11 abrogates its proapoptotic nature and the repression of Smad7. In conclusion, the evidence was given that the transcription factor Klf11 is a downstream mediator of TGF-β-induced apoptosis and a potent modulator of the TGF-β signaling pathway in the oligodendroglial cell line Oli-neu. In a second part of this thesis it has been shown by using semiquantitative PCR and immunoblotting, that nerve growth factor (NGF), a member of the neurotrophin family, regulates the expression of Klf10 in the pheochromocytoma cell line PC12 in a tropomyosin related kinase A- (TrkA) dependent manner. Moreover, the thesis provides evidence for the existence of NGF-responsive elements in the 5	de
dc.contributor.coReferee	Knorr, Christoph Prof. Dr.	de
dc.contributor.thirdReferee	Krieglstein, Kerstin Prof. Dr.	de
dc.subject.topic	Agricultural Sciences	de
dc.subject.ger	Klf10	de
dc.subject.ger	Klf11	de
dc.subject.ger	Sp1-/Krüppelähnliche Transkriptionsfaktoren	de
dc.subject.ger	Apoptose	de
dc.subject.ger	Zellzyklusarrest	de
dc.subject.ger	TGF-β	de
dc.subject.ger	NGF	de
dc.subject.ger	Oli-neu-Zellen	de
dc.subject.ger	PC12-Zellen	de
dc.subject.eng	Klf10	de
dc.subject.eng	KLF11	de
dc.subject.eng	Sp1-/Krüppelähnliche transcription factors	de
dc.subject.eng	apoptosis	de
dc.subject.eng	cell cycle arrest	de
dc.subject.eng	TGF-β	de
dc.subject.eng	NGF	de
dc.subject.eng	Oli-neu-cells	de
dc.subject.eng	PC12-cells	de
dc.subject.bk	???	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3793-8	de
dc.identifier.purl	webdoc-3793	de
dc.affiliation.institute	Fakultät für Agrarwissenschaften	de
dc.identifier.ppn	733998909	de

Dateien

Name:spittau.pdf

Größe:1.635Mb

Format:PDF

Öffnen

Name:: spittau.pdf
Größe:: 1.635Mb
Format:: PDF

Öffnen

Das Dokument erscheint in:

Fakultät für Agrarwissenschaften (inkl. GAUSS) [873]

Zur Kurzanzeige