| dc.contributor.advisor | Feußner, Ivo Prof. Dr. | de |
| dc.contributor.author | König, Stefanie | de |
| dc.date.accessioned | 2013-01-14T15:06:49Z | de |
| dc.date.available | 2013-01-30T23:50:54Z | de |
| dc.date.issued | 2012-10-02 | de |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-EF75-8 | de |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-1456 | |
| dc.description.abstract | Verticillium longisporum ist ein
bodenbürtiger, phytopathogener Pilz, der Pflanzen der Familie der
Brassicaceen befällt. Er dringt durch die Wurzel ein und verbreitet
sich in der Pflanze über das Xylem. In dieser Arbeit wurden die
metabolischen Veränderungen in der Modellpflanze Arabidopsis
thaliana während der Pflanzen-Pilz-Interaktion analysiert. Hierfür
wurde die | de |
| dc.format.mimetype | application/pdf | de |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
| dc.title | Phenylpropanoids and long chain fatty acid derivatives in the interaction of <i>Arabidopsis thaliana</i> and <i>Verticillium longisporum</i> | de |
| dc.type | doctoralThesis | de |
| dc.title.translated | Phenylpropanoide und langkettige Fettsäurederivate in der Interaktion von <i>Arabidopsis thaliana</i> und <i>Verticillium longisporum</i> | de |
| dc.contributor.referee | Feußner, Ivo Prof. Dr. | de |
| dc.date.examination | 2011-10-14 | de |
| dc.subject.dnb | 570 Biowissenschaften, Biologie | de |
| dc.subject.gok | WVE 200 Biochemie | de |
| dc.subject.gok | WVN 000 Pflanzenpathologie | de |
| dc.description.abstracteng | Verticillium longisporum is a soil borne
pathogen which infects plants of the Brassicaceae family. The
fungus enters through the roots and spreads within the plant
through the xylem. Using the model plant Arabidopsis thaliana this
thesis focused on metabolic changes accompanying the plant-fungus
interaction. A metabolite fingerprinting approach was used to find
metabolic changes in Arabidopsis upon infection. The most prominent
markers that were identified derived from the phenylpropanoid
pathway. Quantification of the phenylpropanoids by directed
measurements could confirm the data of the non-targeted approach
showing an accumulation of sinapoyl glucose, coniferin and diverse
lignans already at early stages of infection. To test the
contribution of the identified metabolic pathway on susceptibility
of Arabidopsis against V. longisporum, different mutants of the
phenylpropanoid pathway were analyzed. One of those was a
ferulate-5-hydroxylase mutant (fah1-2) which is devoid of sinapate
and its esters. This mutant showed a higher susceptibility towards
the fungus, underlining the importance of the missing metabolites
for the plant during infection. In addition a coniferin
accumulation mutant (UGT72E2-OE) showed less stunting and more
fungal DNA than wild type plants under infection. This effect might
be due to fungal growth inhibiting properties of the deglucosylated
coniferin or to an influence on fungal development of coniferin
itself.
Additionally implication of suberin and sphingolipids with
α-hydroxy fatty acids in the infection was tested in this thesis.
Suberin analyses at early time points of infection as well as
infection of suberin mutants did not reveal any importance of this
polymer in the infection. To investigate the functional
significance of the α-hydroxylation in fatty acids of
sphingolipids, T-DNA insertion mutants of both Fatty Acid
Hydroxylase genes (AtFAH1 and AtFAH2) were analyzed in Arabidopsis.
No phenotype was visible in the single mutant lines but the double
mutant showed reduction in leaf size, root length and wrinkled
leaves. Ceramide and glucosylceramide profiles of the double mutant
showed a strong reduction of sphingolipids with α-hydroxylated
fatty acid moieties and an accumulation of the one without hydroxy
group. The total ceramide amount was ten times increased in the
double mutant, whereas the glucosylceramide pool was 25 % reduced.
Metabolite fingerprinting of the double mutant revealed differences
compared to wild type in the metabolome. The most prominent markers
were salicylic acid (SA) and its glucoside. Infection of the double
mutant with powdery mildew resulted in less fungal colonization
whereas infection with V. longisporum resulted in stronger stunting
and slightly higher fungal DNA amount in infected plants.
In summary it could be shown that the phenylpropanoid pathway is
important for the defense response in Arabidopsis against V.
longisporum but fatty acid monomers derived from suberin and
sphingolipids seem to play no or only a minor role. | de |
| dc.contributor.coReferee | Dröge-Laser, Wolfgang Prof. Dr. | de |
| dc.subject.topic | Biology (incl. Psychology) | de |
| dc.subject.ger | Verticillium longisporum | de |
| dc.subject.ger | Arabidopsis thaliana | de |
| dc.subject.ger | Phenylpropanoide | de |
| dc.subject.ger | Sphingolipide | de |
| dc.subject.ger | Fettsäure-Hydroxylase | de |
| dc.subject.ger | Suberin | de |
| dc.subject.eng | Verticillium longisporum | de |
| dc.subject.eng | Arabidopsis thaliana | de |
| dc.subject.eng | phenylpropanoids | de |
| dc.subject.eng | sphingolipids | de |
| dc.subject.eng | fatty acid hydroxylase | de |
| dc.subject.eng | suberin | de |
| dc.subject.bk | 42.41 Pflanzenphysiologie | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-3694-8 | de |
| dc.identifier.purl | webdoc-3694 | de |
| dc.affiliation.institute | Biologische Fakultät | de |
| dc.identifier.ppn | 737897597 | de |
| dc.creator.birthname | Götze | |