Regulation der Aktivität und Lokalisation von Antiterminatorproteinen der BglG-Familie

Rothe, Fabian

dc.contributor.advisor	Stülke, Jörg Prof. Dr.	de
dc.contributor.author	Rothe, Fabian	de
dc.date.accessioned	2013-01-14T15:06:51Z	de
dc.date.available	2013-01-30T23:51:03Z	de
dc.date.issued	2012-11-12	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-EF83-8	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-1457
dc.description.abstract	Die Regulation der Genexpression ist essentiell für alle Bakterien und ermöglicht die Anpassung an sich verändernde Lebensbedingungen. Verschiedene Transkriptions- und Translationsfaktoren haben bei dieser Regulation eine große Bedeutung. Die Antiterminationsproteine der BglG/ SacY-Familie sind in Bakterien weit verbreitet und steuern die Expression von Genen und Operons, die an der Aufnahme und Verwertung von Kohlenstoffquellen beteiligt sind. Die substrat- und phosphorylierungsabhängige Aktivierung der Proteine der BglG/ SacY-Familie erlaubt es ihnen, an ihre Ziel-RNA zu binden und ermöglicht das Fortschreiten der blockierten Transkription. Der Transkriptionsfaktor BglG aus Escherichia coli ist	de
dc.format.mimetype	application/pdf	de
dc.language.iso	ger	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	Regulation der Aktivität und Lokalisation von Antiterminatorproteinen der BglG-Familie	de
dc.type	doctoralThesis	de
dc.title.translated	Regulation of the Activity and Localization of BglG family Antiterminator Proteins	de
dc.contributor.referee	Stülke, Jörg Prof. Dr.	de
dc.date.examination	2012-03-23	de
dc.subject.dnb	570 Biowissenschaften, Biologie	de
dc.subject.gok	WUE 200	de
dc.subject.gok	WUK 000	de
dc.subject.gok	WJL 200	de
dc.description.abstracteng	The regulation of gene expression is essential for all bacteria and allows adapting to changing life conditions. Different transcription and translation factors have an important impact on gene regulation. The antermination proteins of the BglG/ SacY family are widely spread. They control the expression of genes and operons which are involved in the uptake and utilization of different carbon sources. The substrate and phosphorylation dependent activation of the BglG/ SacY family proteins enable these antiterminators to bind their target RNA and allow proceeding the blocked transcription. The transcription factor BglG of Escherichia coli is equivalent to LicT of Bacillus subtilis. Nobody has yet found out whether these two antitermination proteinsare identical and if they have the same function in both organisms. In contrast to LicT, the activation mechanism of BglG is not fully answered yet. Compared to BglG the localization of active and inactive LicT is also unclear. In this work it was shown that BglG is phosphorylated and activated by HPr or FruB which is a homolog to HPr. Furthermore, the phosphorylation dependent dynamic localization of LicT was detected. In vitro experiments could show that BglG is phosphorylated by HPr (or FruB) and thereby activated at histidine-208. Moreover, fluorescence microscopy detected, that inactive LicT (in the absence of beta-glucosides) is constantly distributed in the cytoplasm and accumulates in the cell poles in its active state (after the addition of beta-glucosides). Mutation analysis of the conserved phosphorylation sites of LicT revealed that the state of phosphorylation has an influence on the dynamic localization of LicT regardless of the LicT activity. The results show that the control mechanism of E. coli BglG is identical to B. subtilis LicT and to the other members of BglG / SacY family. Moreover, it was found that the LicT controlled regulation of gene expression is more than a simple mechanism, but rather a highly dynamic process. These observations suggest that there must be more dynamic localization processes among the many regulatory mechanisms. Further studies may elucidate those factors which influence post-translational modifications and the dynamic protein localization.	de
dc.contributor.coReferee	Tittmann, Kai Prof. Dr.	de
dc.contributor.thirdReferee	Seiler, Stephan PD Dr.	de
dc.subject.topic	Biology (incl. Psychology)	de
dc.subject.ger	LicT	de
dc.subject.ger	BglG	de
dc.subject.ger	Phosphorylierung	de
dc.subject.ger	Antitermination	de
dc.subject.ger	RNA-Regulation	de
dc.subject.ger	Escherichia coli	de
dc.subject.ger	Bacillus subtilis	de
dc.subject.ger	dynamische Proteinlokalisation	de
dc.subject.ger	Phosphotransferasesystem	de
dc.subject.ger	Genregulation	de
dc.subject.ger	FruB	de
dc.subject.ger	HPr	de
dc.subject.eng	LicT	de
dc.subject.eng	BglG	de
dc.subject.eng	phosphorylation	de
dc.subject.eng	antiterminator	de
dc.subject.eng	RNA regulation	de
dc.subject.eng	Escherichia coli	de
dc.subject.eng	Bacillus subtilis	de
dc.subject.eng	dynamic protein localisation	de
dc.subject.eng	phosphotransferase system	de
dc.subject.eng	gene regulation	de
dc.subject.eng	FruB	de
dc.subject.eng	HPr	de
dc.subject.bk	42.30	de
dc.subject.bk	42.13	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3789-6	de
dc.identifier.purl	webdoc-3789	de
dc.affiliation.institute	Biologische Fakultät	de
dc.identifier.ppn	73131235X	de

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