dc.contributor.advisor | Kugler, Wilfried PD Dr. | de |
dc.contributor.author | Köhler, Franziska | de |
dc.date.accessioned | 2013-01-14T15:27:12Z | de |
dc.date.available | 2013-01-30T23:50:53Z | de |
dc.date.issued | 2012-07-05 | de |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-EFDD-2 | de |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-1566 | |
dc.format.mimetype | application/pdf | de |
dc.language.iso | ger | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
dc.title | Etablierung und Validierung der RNA-Interferenzmethode am Beispiel Apoptose-relevanter Gene | de |
dc.type | doctoralThesis | de |
dc.title.translated | Establishment and validation of RNA interference using the example of apoptosis-relevant genes | de |
dc.contributor.referee | Lakomek, Max Prof. Dr. | de |
dc.date.examination | 2012-07-24 | de |
dc.subject.dnb | 610 Medizin, Gesundheit | de |
dc.subject.gok | MED 000 | de |
dc.description.abstracteng | Specific inhibition of gene expression is
becoming an increasingly important method for experimental
strategies to influence tumor growth on a molecular level. In this
work, we investigated the role of individual molecules for
erucylphosphocholin (ErPC) induced apoptosis using specific,
synthesized small interfering RNAs (siRNAs).
The human gliobastoma cell lines A172, U118MG and U373MG were used
as model system; protein expression was measured with western blot
analysis. As a first step, we confirmed that the RNA interference
method is in fact applicable to our in vitro model system for human
brain tumor cells using a simple, morphological test. In the second
step, we optimzied the RNA interference method for our model cell
lines by targeting the mRNA of the cytoskeleton protein Lamin A/C.
The transfection parameters optimized were the cell count and the
concentrations of siRNA and transfection reagent Oligogectamin. The
specificity of the employed method was confirmed using a control
siRNA targeting Luciferase.
In the third step, we applied the RNA interference method employing
our optimized parameters to the genes APAF-1, Caspase 2 and Caspase
3, which are known to participate in cell apoptosis. We were able
to show that the expression of the respective proteins can be down
regulated in different glioblastoma cell lines using specific
siRNA.
The last part of this work investigates the functional importance
of Caspase 3 and APAF-1 “knock down | de |
dc.contributor.coReferee | Bastians, Holger Prof. Dr. | de |
dc.contributor.thirdReferee | Hanisch, Uwe-Karsten Prof. Dr. | de |
dc.subject.topic | Medicine | de |
dc.subject.ger | RNA-Interferenz | de |
dc.subject.ger | Apoptose | de |
dc.subject.ger | Erucylphosphocholin | de |
dc.subject.ger | Caspase 3 | de |
dc.subject.ger | Caspase 2 | de |
dc.subject.ger | Apaf-1 | de |
dc.subject.ger | Glioblastomzellen | de |
dc.subject.eng | siRNA | de |
dc.subject.eng | apoptosis | de |
dc.subject.eng | erucylphosphocholine | de |
dc.subject.eng | caspase-3 | de |
dc.subject.eng | Caspase-2 | de |
dc.subject.eng | apaf-1 | de |
dc.subject.eng | glioma | de |
dc.subject.bk | 44.00 | de |
dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-3602-3 | de |
dc.identifier.purl | webdoc-3602 | de |
dc.affiliation.institute | Medizinische Fakultät | de |
dc.identifier.ppn | 728919729 | de |