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dc.contributor.advisor Steinem, Claudia Prof. Dr. de
dc.contributor.author Carnarius, Christian de
dc.date.accessioned 2012-11-19T15:44:04Z de
dc.date.accessioned 2013-01-18T10:39:11Z de
dc.date.available 2013-01-30T23:51:01Z de
dc.date.issued 2012-11-19 de
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-000D-F053-F de
dc.format.mimetype application/pdf de
dc.language.iso ger de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ de
dc.title Funktionelle Rekonstitution von Connexonen in artifizielle Membranen: Expression, Reinigung und Charakterisierung von Connexin 43 de
dc.type doctoralThesis de
dc.title.translated Functional reconstitution of connexons in artificial membranes: expression, purification and characterization of connexin 43 de
dc.contributor.referee Steinem, Claudia Prof. Dr. de
dc.date.examination 2012-06-11 de
dc.subject.dnb 570 Biowissenschaften de
dc.subject.dnb Biologie de
dc.subject.gok SX 000 de
dc.subject.gok WC 000 de
dc.subject.gok WR 000 de
dc.subject.gok SXH 000 de
dc.description.abstracteng Connexin 43 (Cx43) is the most prominent member of the connexin family in mammalian tissues. In biological lipid membranes, six connexin subunits assemble into a hemichannel (connexon). Direct communication of apposing cells is realized by two adjacent hemichannels, forming gap junction channels. Membrane channel proteins are pivotal for communication between cells. Dysfunctional ion channels are associated to diseases like osteoporosis, epilepsy, ataxia, myotonia and cardiac arrhythmia. Therefore, there is an interest in studying these channel proteins to elucidate their functional properties and determine how to manipulate their functionality. This is commonly carried out by ion channel recordings, which require invasive contacting by voltage clamp methods in cells. The alternative approach taken here was to utilize connexon-doped artificial membranes for electrical coupling of connexon-expressing cells via gap junction channels. In this work, an expression system in Pichia pastoris to recombinantly produce and purify Cx43 as well as Cx43 fused to green fluorescent protein (Cx43 de
dc.contributor.coReferee Griesinger, Christian Prof. Dr. de
dc.subject.topic Chemistry de
dc.subject.ger Connexin 43 de
dc.subject.ger Cx43 de
dc.subject.ger Grün fluoreszierendes Protein de
dc.subject.ger GFP de
dc.subject.ger Porenüberspannende Membranen de
dc.subject.ger Poröses Aluminiumoxid de
dc.subject.ger Artifizielle Membranen de
dc.subject.ger Planar Patch Clamp de
dc.subject.ger Melittin de
dc.subject.ger Pichia pastoris de
dc.subject.ger D. discoideum de
dc.subject.eng connexin 43 de
dc.subject.eng Cx43 de
dc.subject.eng green fluorescent protein de
dc.subject.eng GFP de
dc.subject.eng pore spanning membranes de
dc.subject.eng porous alumina de
dc.subject.eng artificial membranes de
dc.subject.eng planar patch clamp de
dc.subject.eng melittin de
dc.subject.eng Pichia pastoris de
dc.subject.eng Dictyostelium discoideum de
dc.subject.bk 42.12 de
dc.subject.bk 35.78 de
dc.subject.bk 35.71 de
dc.identifier.urn urn:nbn:de:gbv:7-webdoc-3797-1 de
dc.identifier.purl webdoc-3797 de
dc.affiliation.institute Fakultät für Chemie de
dc.identifier.ppn 737898712 de

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