Synaptic vesicle recycling investigated by high-resolution microscopy in a conventional and a sensory synapse

Kamin, Dirk

dc.contributor.advisor	Rizzoli, Silvio Prof. Dr.	de
dc.contributor.author	Kamin, Dirk	de
dc.date.accessioned	2012-10-02T18:36:09Z	de
dc.date.accessioned	2013-01-18T14:24:23Z	de
dc.date.available	2013-01-30T23:50:18Z	de
dc.date.issued	2012-10-02	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-F0BF-1	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3212
dc.format.mimetype	application/pdf	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	Synaptic vesicle recycling investigated by high-resolution microscopy in a conventional and a sensory synapse	de
dc.type	doctoralThesis	de
dc.title.translated	Das synaptische Vesikelrecycling untersucht durch hochauflösende Mikroskopie in einer konventionellen und einer sensorischen Synapse	de
dc.contributor.referee	Rizzoli, Silvio Prof. Dr.	de
dc.date.examination	2011-04-15	de
dc.subject.dnb	570 Biowissenschaften	de
dc.subject.dnb	Biologie	de
dc.subject.gok	WA 310	de
dc.subject.gok	WHC 500	de
dc.subject.gok	WXG 900	de
dc.description.abstracteng	Synaptic neurotransmission depends on the action of highly specialized small secretory organelles – synaptic vesicles. At all chemical synapses the synaptic vesicles release in a strictly regulated fashion their neurotransmitter into the synaptic cleft, by fusing with the plasma membrane (exocytosis) at structurally and functionally well organized sites (active zones). The fused vesicles get retrieved (endocytosis), refilled with neurotransmitter and returned to the vesicle cluster near the active zone in order to supply fusion-competent vesicles for further rounds of neurotransmission, thus completing a process termed vesicle recycling. While the molecular key players in the synaptic vesicle cycle have been characterized in detail for conventional synapses, the general mobility of synaptic vesicles is still poorly understood, with only averaged behaviors (over populations of vesicles) known. How could vesicle behavior actually be described when single vesicles are analyzed? The small size of synaptic vesicles (~ 40 nm in diameter) and the dense clustering at the active zone makes it difficult to study their behavior, since conventional imaging techniques are restricted to a resolution of ~200-300 nm by the diffraction limit of light. I investigated here synaptic vesicle mobility throughout the synaptic vesicle cycle, using both conventional and sub-diffraction high-resolution stimulated emission depletion (STED) fluorescence microscopy. I obtained a thorough description of vesicle recycling in hippocampal synapses. Single vesicle tracking revealed that a large resting pool of vesicles has a low mobility, while active, recently endocytosed vesicles exhibit a high-mobility state for a substantial amount of time. They eventually become resting vesicles by integrating into the vesicle cluster (“maturation	de
dc.contributor.coReferee	Moser, Tobias Prof. Dr.	de
dc.subject.topic	Göttingen Graduate School for Neurosciences and Molecular Biosciences (GGNB)	de
dc.subject.ger	STED Mikroskopie	de
dc.subject.ger	Elektronenmikroskopie	de
dc.subject.ger	Photo-oxidation	de
dc.subject.ger	FM Farbstoff	de
dc.subject.ger	synaptisches Vesikel	de
dc.subject.ger	Vesikelrecycling	de
dc.subject.ger	Exozytose	de
dc.subject.ger	Endozytose	de
dc.subject.ger	Cortisches Organ	de
dc.subject.ger	Bändersynapse	de
dc.subject.ger	innere Haarzelle	de
dc.subject.eng	STED microscopy	de
dc.subject.eng	electron microscopy	de
dc.subject.eng	photo-oxidation	de
dc.subject.eng	FM dye	de
dc.subject.eng	synaptic vesicle	de
dc.subject.eng	vesicle recycling	de
dc.subject.eng	exocytosis	de
dc.subject.eng	endocytosis	de
dc.subject.eng	organ of corti	de
dc.subject.eng	ribbon synapse	de
dc.subject.eng	inner hair cell	de
dc.subject.bk	42.03	de
dc.subject.bk	42.15	de
dc.subject.bk	42.63	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3725-6	de
dc.identifier.purl	webdoc-3725	de
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften und Molekulare Biowissenschaften (GGNB)	de
dc.identifier.ppn	737899433	de

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Das Dokument erscheint in:

GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1115]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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