Exploring the neural basis of touch through selective and stable genetic tagging in the chick somatosensory system

Cyganek, Lukas

dc.contributor.advisor	Marquardt, Till Prof. Dr.	de
dc.contributor.author	Cyganek, Lukas	de
dc.date.accessioned	2013-01-03T18:37:37Z	de
dc.date.accessioned	2013-01-18T14:28:00Z	de
dc.date.available	2013-06-19T22:50:04Z	de
dc.date.issued	2013-01-03	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-F0CB-5	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3282
dc.format.mimetype	application/pdf	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	Exploring the neural basis of touch through selective and stable genetic tagging in the chick somatosensory system	de
dc.type	doctoralThesis	de
dc.contributor.referee	Marquardt, Till Prof. Dr.	de
dc.date.examination	2012-12-20	de
dc.subject.dnb	570 Biowissenschaften	de
dc.subject.dnb	Biologie	de
dc.subject.gok	Molekularbiologie (PPN61946299X)	de
dc.description.abstracteng	The assembly of circuitries for somatosensory perception relies on a complex interplay of neuronal diversification, specification, phenotypic modulation and establishment of precise connectivity patterns during embryogenesis. However, little is known about the molecular mechanisms controlling the establishment of well-defined somatosensory connectivity, which is to a large part due to the limitation of sensory neuron subtype-specific molecular markers. This study aimed at the identification of novel genetic markers for distinct somatosensory neuron subtypes to provide more detailed insights into primary somatosensory circuit formation, organization and function. These aims were addressed by a newly established in silico-to-in vivo screen for neuron subtype-specific enhancer activities in the chick at late-gestation stages. This study designed a simple screening strategy that permits rapid, efficient and stable genetic tagging of sensory neuron subtypes in vivo, which thus provides the basis for the systematic discovery of late-onset sensory neuron subtype-specific gene regulatory activities. Thereby, a novel molecularly defined subclass of touch receptor neurons was uncovered, thus providing the utility of the system for uncovering the neural basis of discrete sensory modalities relayed by the primary somatosensory system. In addition, the system facilitated stable cell fate-tracking, which unraveled outstanding features of the primary somatosensory neuron lineage. Besides facilitating the genetic dissection of the somatosensory system, the strategy also offers an effective pre-screening platform for targeting genetically identified neuronal subtypes in other vertebrate species.	de
dc.contributor.coReferee	Eichele, Gregor Prof. Dr.	de
dc.contributor.thirdReferee	Nave, Klaus-Armin Prof. Dr.	de
dc.subject.topic	Göttingen Graduate School for Neurosciences and Molecular Biosciences (GGNB)	de
dc.subject.eng	touch perception	de
dc.subject.eng	somatosensory circuit connectivity	de
dc.subject.eng	sensory neuron subtypes	de
dc.subject.eng	enhancer identification	de
dc.subject.eng	late-gestation chick embryos	de
dc.subject.bk	42.13 Molekularbiologie	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3854-3	de
dc.identifier.purl	webdoc-3854	de
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften und Molekulare Biowissenschaften (GGNB)	de
dc.identifier.ppn	773354980

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Das Dokument erscheint in:

GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1111]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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