dc.contributor.advisor | Hoyer-Fender, Sigrid PD Dr. | de |
dc.contributor.author | Donkor, Fatima | de |
dc.date.accessioned | 2013-01-22T15:38:00Z | de |
dc.date.available | 2013-01-30T23:51:00Z | de |
dc.date.issued | 2008-02-18 | de |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-F119-B | de |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-3391 | |
dc.format.mimetype | application/pdf | de |
dc.language.iso | ger | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
dc.title | Interaktionen des testikulären outer dense fibre protein 2 (ODF 2)bei der zellulären Organisation | de |
dc.type | doctoralThesis | de |
dc.title.translated | Interactions of the testicular outer dense fibre protein 2 (ODF 2) during cellular organisation | de |
dc.contributor.referee | Engel, W. Prof. Dr. | de |
dc.date.examination | 2006-10-31 | de |
dc.subject.dnb | 570 Biowissenschaften, Biologie | de |
dc.subject.gok | WK | de |
dc.description.abstracteng | Outer dense fibre 2 was initially
identified as a main component of the sperm tail cy-toskeleton and
was shown as a self-interacting centrosomal protein in somatic
cells with affinity for microtubules. ODF2 has different
localization in somatic versus male germ cells suggesting the
possi-bility of different function. It was an aim to understand how
the testicular protein ODF2 is involved in the cellular
organisation of somatic cells and at the same time to identify
puta-tive interaction partners which eventually take part in this
process.
A yeast two hybrid screen enabled to identify putative ODF2
interaction partners which are expected to be involved in protein
transport, microtubuli binding and apoptotic proc-esses. The known
ODF2 interactive partner ODF1 (Shao et al., 1997) co localized in
somatic cells with centrosomal structures. Odf2/Odf1 cellular
co-transfection experiments demonstrate a fibrillar molecular
protein network generated by ODF1-ODF2 interaction event. ODF2 and
dynamitin-overexpressing cells indicated an involvement of ODF2 in
dynein-dependent protein assembly. ODF2 associated in somatic cells
with the endoplasmtic reticulum and the Golgi complex.
Immuncytology experiments with truncated ODF2 GFP fusion proteins
suggested that the C terminal domain of ODF2 is thereby involved in
binding to these specific organelles. Time series incubation of
cells in culture with Brefeldin A (BFA) to analyse intracellular
protein transport on ODF2 overexpressing cells were performed.
Cells lead to similar but not identical results. Thus, the effects
of short-term treatment with BFA was different from those of
long-term treatment.
Proliferation assays and flow cytometry analyses demonstrated that
ODF2 overexpress-ing cells lead to a significant decrease of
proliferation by “losing | de |
dc.contributor.coReferee | Feussner, Ivo Prof. Dr. | de |
dc.contributor.thirdReferee | Grossbach, Ulrich Prof. Dr. | de |
dc.subject.topic | Mathematics and Natural Science | de |
dc.subject.ger | Outer dense fibre Proteine | de |
dc.subject.ger | Centrosom | de |
dc.subject.ger | ODF2 | de |
dc.subject.ger | ODF1 | de |
dc.subject.eng | Outer dense fibre protein | de |
dc.subject.eng | centrosom | de |
dc.subject.eng | ODF2 | de |
dc.subject.eng | ODF1 | de |
dc.subject.bk | 42.13 | de |
dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-1713-9 | de |
dc.identifier.purl | webdoc-1713 | de |
dc.identifier.ppn | 591104156 | de |