| dc.contributor.advisor | Polle, Andrea Prof. Dr. | de |
| dc.contributor.author | Floerl, Saskia | de |
| dc.date.accessioned | 2013-01-22T15:40:02Z | de |
| dc.date.available | 2013-01-30T23:50:58Z | de |
| dc.date.issued | 2008-03-04 | de |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-F12A-5 | de |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-3408 | |
| dc.format.mimetype | application/pdf | de |
| dc.language.iso | ger | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
| dc.title | Identifizierung und Charakterisierung extrazellulärer Proteine unter dem Einfluss von Verticillium longisporum in Arabidopsis thaliana und Raps (<i>Brassica napus</i>) | de |
| dc.type | doctoralThesis | de |
| dc.title.translated | Identification and characterization of extracellular proteins in Arabidopsis thaliana and <i>Brassica napus</i> | de |
| dc.contributor.referee | Gatz, Christiane Prof. Dr. | de |
| dc.date.examination | 2007-11-01 | de |
| dc.subject.dnb | 570 Biowissenschaften, Biologie | de |
| dc.subject.gok | WA 340 | de |
| dc.description.abstracteng | Verticillium species are soil-borne,
xylem-colonizing fungi with worldwide distribution in temperate and
subtropical regions. They cause vascular disease in many
agriculturally important crops. Verticillium longisporum is
specialized on Brassicaceae e. g. cauliflower, turnip and rapeseed.
In europe V. longisporum became an increasing problem with the
growing area of oilseed rape production.
This thesis aims to extend the knowledge about the interaction of
V. longisporum and its host plants. The major goal was the
characterization of the extracellular proteome of Arabidopsis
thaliana and Brassica napus influenced by V.longisporum and in this
context the description of infection symptoms. B. napus was chosen
as example for an economically important crop, while the model
plant A. thaliana provided the possibility for molecular
analysis.
The characterization of infection symptoms showed a reduced A.
thaliana rosette area and a reduced shoot height with B. napus. In
leaves of both plant species the chlorophyll content and activity
of photosystem II were reduced by V. longisporum infection.
Quantification of Verticillium-DNA by real time PCR resulted in no
direct interrelation between symptome development and amount of
fungus in overground plant parts, a hint that not the direct
contact with the fungus but direct or indirect signals lead to the
symptome development. Electrolyte leakage measurements showed that
cell membranes were not demaged by the fungus at the timepoint of
proteome analysis.
Soluble apoplastic and xylem sap proteins from V. longisporum
inoculated A. thaliana and B. napus leaves were separated by one-
and two-dimensional electrophoresis. With both methods we observed
differences in the protein pattern of Verticillium-inoculated and
control plants. Proteins were identified by mass spectrometry and
NCBI database search. In the leaf apoplast of V. longisporum
infected A. thaliana a serine carboxypeptidase, an a-galactosidase,
a germin-like protein and three peroxidases with increased
expression and a lectin-like protein with decreased expression were
found. The results for A. thaliana proteome analysis were verified
by quantitative real time PCR. The B. napus apoplast under V.
longisporum influence showed increased expression of a glucanase, a
chitinase, a peroxidase and a PR-4 proteine. The glucanase and the
PR-4 proteine also occurred in xylem sap with increased expression.
Because increased expression of chitinases were often described in
connection with Verticillium infection, here also in B. napus, the
absence of increasingly expressed chitinases in the A. thaliana
apoplast was amazing. Genexpression analysis for an acidic
endochitinase (At5g24090) showed an obviously increased expression
post V. longisporum infection. Though the overexpression of this
gene didn | de |
| dc.contributor.coReferee | Behrens, Susanne Dr. | de |
| dc.contributor.thirdReferee | Liebl, Wolfgang Prof. Dr. | de |
| dc.subject.topic | Mathematics and Natural Science | de |
| dc.subject.bk | 35.70 Biochemie: Allgemeines | de |
| dc.subject.bk | 42.42 (Pflanzenphysiologie) | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-1730-3 | de |
| dc.identifier.purl | webdoc-1730 | de |
| dc.identifier.ppn | 588951579 | de |