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dc.contributor.advisor Polle, Andrea Prof. Dr. de
dc.contributor.author Floerl, Saskia de
dc.date.accessioned 2013-01-22T15:40:02Z de
dc.date.available 2013-01-30T23:50:58Z de
dc.date.issued 2008-03-04 de
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-000D-F12A-5 de
dc.format.mimetype application/pdf de
dc.language.iso ger de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ de
dc.title Identifizierung und Charakterisierung extrazellulärer Proteine unter dem Einfluss von Verticillium longisporum in Arabidopsis thaliana und Raps (<i>Brassica napus</i>) de
dc.type doctoralThesis de
dc.title.translated Identification and characterization of extracellular proteins in Arabidopsis thaliana and <i>Brassica napus</i> de
dc.contributor.referee Gatz, Christiane Prof. Dr. de
dc.date.examination 2007-11-01 de
dc.subject.dnb 570 Biowissenschaften, Biologie de
dc.subject.gok WA 340 de
dc.description.abstracteng Verticillium species are soil-borne, xylem-colonizing fungi with worldwide distribution in temperate and subtropical regions. They cause vascular disease in many agriculturally important crops. Verticillium longisporum is specialized on Brassicaceae e. g. cauliflower, turnip and rapeseed. In europe V. longisporum became an increasing problem with the growing area of oilseed rape production. This thesis aims to extend the knowledge about the interaction of V. longisporum and its host plants. The major goal was the characterization of the extracellular proteome of Arabidopsis thaliana and Brassica napus influenced by V.longisporum and in this context the description of infection symptoms. B. napus was chosen as example for an economically important crop, while the model plant A. thaliana provided the possibility for molecular analysis. The characterization of infection symptoms showed a reduced A. thaliana rosette area and a reduced shoot height with B. napus. In leaves of both plant species the chlorophyll content and activity of photosystem II were reduced by V. longisporum infection. Quantification of Verticillium-DNA by real time PCR resulted in no direct interrelation between symptome development and amount of fungus in overground plant parts, a hint that not the direct contact with the fungus but direct or indirect signals lead to the symptome development. Electrolyte leakage measurements showed that cell membranes were not demaged by the fungus at the timepoint of proteome analysis. Soluble apoplastic and xylem sap proteins from V. longisporum inoculated A. thaliana and B. napus leaves were separated by one- and two-dimensional electrophoresis. With both methods we observed differences in the protein pattern of Verticillium-inoculated and control plants. Proteins were identified by mass spectrometry and NCBI database search. In the leaf apoplast of V. longisporum infected A. thaliana a serine carboxypeptidase, an a-galactosidase, a germin-like protein and three peroxidases with increased expression and a lectin-like protein with decreased expression were found. The results for A. thaliana proteome analysis were verified by quantitative real time PCR. The B. napus apoplast under V. longisporum influence showed increased expression of a glucanase, a chitinase, a peroxidase and a PR-4 proteine. The glucanase and the PR-4 proteine also occurred in xylem sap with increased expression. Because increased expression of chitinases were often described in connection with Verticillium infection, here also in B. napus, the absence of increasingly expressed chitinases in the A. thaliana apoplast was amazing. Genexpression analysis for an acidic endochitinase (At5g24090) showed an obviously increased expression post V. longisporum infection. Though the overexpression of this gene didn de
dc.contributor.coReferee Behrens, Susanne Dr. de
dc.contributor.thirdReferee Liebl, Wolfgang Prof. Dr. de
dc.subject.topic Mathematics and Natural Science de
dc.subject.bk 35.70 Biochemie: Allgemeines de
dc.subject.bk 42.42 (Pflanzenphysiologie) de
dc.identifier.urn urn:nbn:de:gbv:7-webdoc-1730-3 de
dc.identifier.purl webdoc-1730 de
dc.identifier.ppn 588951579 de

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