• Deutsch
    • English
  • English 
    • Deutsch
    • English
  • Login
Item View 
  •   Home
  • Naturwissenschaften, Mathematik und Informatik
  • Fakultät für Physik (inkl. GAUSS)
  • Item View
  •   Home
  • Naturwissenschaften, Mathematik und Informatik
  • Fakultät für Physik (inkl. GAUSS)
  • Item View
JavaScript is disabled for your browser. Some features of this site may not work without it.

3D STED Microscopy with Pulsed and Continuous Wave Lasers

3D STED Mikroskopie mit gepulsten und Dauerstrichlasern

by Benjamin Harke
Doctoral thesis
Date of Examination:2008-04-02
Date of issue:2008-04-16
Advisor:Prof. Dr. Stefan Hell
Referee:Prof. Dr. Stefan Hell
Referee:Prof. Dr. Markus Münzenberg
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-3436

 

 

Files in this item

Name:harke.pdf
Size:21.0Mb
Format:PDF
Description:Dissertation
ViewOpen

The following license files are associated with this item:


Abstract

English

STED microscopy improves the resolution of fluorescence microscope by effectively quenching the population of the excited state in the outer regions of the focus. The resolution capability in STED microscopy has already been shown in optical sections particularly in the lateral directions. Biological samples have been prepared in a manner, that the resolution enhancement in the lateral plane is sufficient to tackle the biological problem. However, a cell for example is a three dimensional object and though has to be recorded in all spatial directions in order to get information about structural details. The way to three dimensional imaging with an enhanced resolution in all directions will be presented in this work. The influence of different depletion patterns on the resolution of the STED microscope is thereby a very important parameter. A quantitative investigation of the resolution enhancement in a STED microscope will be discussed in the following chapter. The capability of performing three dimensional imaging will be presented in chapter 3. Thereby for the first time the incoherently combination of two depletion patterns - one for the resolution enhancement in the lateral plane and one for the axial direction - will be presented. The resulting minimized extent of the focal spot enables the three dimensional imaging of colloidal crystals with an enhanced resolution in all three directions. Currently the implementation of STED microscopy as a standard imaging tool is mainly inhibited by the complex experimental platform including sophisticated and expensive laser sources as well as electrical equipment. In chapter 4, this thesis shows the first ever use of continuous wave (CW) lasers for the excitation and STED light sources. The simplicity of the setup can facilitate the way to a wide use of STED microscopy allowing in principle every existing scanning fluorescence microscope to perform high resolution.
Keywords: Microscopy; Fluorescence; High resolution; STED

Other Languages

STED Mikroskopie erhöht das Auflösungsvermögen eines Fluoreszenzmikroskops, indem die Bevölkerung des angeregten Zustandes in den äußeren Regionen des Fokus effektiv reduziert wird. Die Auflösungserhöhung in der lateralen Ebene wurde bereits in Publikationen validiert. Viele Proben können in der Weise präpariert werden, dass diese Auflösungserhöhung ausreichend ist. Allerdings gibt es Proben, beispielsweise biologische Strukturen, die eine Auflösungserhöhung in alle 3 Raumrichtungen benötigen, um den Aufbau der Zelle voll aufzulösen. Die Problematik der dreidimensionalen Auflösungserhöhung wird in dieser Arbeit diskutiert. Die Komplexität des daraus resultierenden experimentellen Aufbaus kann durch den Einsatz von Dauerstrichlasern anstatt gepulster Strahlquellen deutlich reduziert werden. Der Einsatz von Dauerstrichlasern in einem STED Mikroskop wird im letzten Kapitel dieser Arbeit präsentiert.
Schlagwörter: Mikroskop; Fluoreszenz; Hochauflösung; STED
 

Statistik

Publish here

Browse

All of eDissFaculties & ProgramsIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesTypeThis FacultyIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesType

Help & Info

Publishing on eDissPDF GuideTerms of ContractFAQ

Contact Us | Impressum | Cookie Consents | Data Protection Information
eDiss Office - SUB Göttingen (Central Library)
Platz der Göttinger Sieben 1
Mo - Fr 10:00 – 12:00 h


Tel.: +49 (0)551 39-27809 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
ediss_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]
Göttingen State and University Library | Göttingen University
Medicine Library (Doctoral candidates of medicine only)
Robert-Koch-Str. 40
Mon – Fri 8:00 – 24:00 h
Sat - Sun 8:00 – 22:00 h
Holidays 10:00 – 20:00 h
Tel.: +49 551 39-8395 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
bbmed_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]