| dc.contributor.advisor | Vorbrüggen, Gerd Dr. | de |
| dc.contributor.author | Chanana, Bhavna | de |
| dc.date.accessioned | 2013-01-22T15:44:49Z | de |
| dc.date.available | 2013-01-30T23:50:58Z | de |
| dc.date.issued | 2008-04-25 | de |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-F150-F | de |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-3446 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-3446 | |
| dc.description.abstract | Syndecan (Sdc) ist ein Transmembranprotein
der Klasse I, das im Bereich seiner extrazellulären Domäne mit
mehreren linearen Zuckerketten modifiziert ist. Diese Zuckerketten
werden als Heparansulfat-Glykosaminoglykane (HSGAG) bezeichnet. In
vorhergehenden Experimenten konnte eine essentielle Funktion des
Sdc-Proteins für die Slit-Signaltransduktion an der Mittellinie des
Drosophila Embryos gezeigt werden. Das sezernierte Slit-Protein
über die Bindung an den Robo-Rezeptor als abstoßende Aktivität, die
sowohl die Axone des zentralen Nervensystems (ZNS) als auch die
Muskulatur im ventralen Bereich des Embryos strukturiert. Die
vorliegende Doktorarbeit hatte das Ziel, ein besseres Verständnis
der Funktion des Sdc-Proteins innerhalb des
Slit/Robo-Signaltransduktionsweges zu gewinnen. Mit Hilfe des
UAS/GAL4-Systems wurden verschiedene Sdc-Proteinvarianten und
Protein | de |
| dc.format.mimetype | application/postscript | de |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
| dc.title | Functional Characterisation of Syndecan, a heparan sulpahte proteoglycan, in Slit/Robo signalling | de |
| dc.type | doctoralThesis | de |
| dc.title.translated | Funktionale Charakterisierung von Syndecan, ein Heparansulfatproteoglykan, im Slit/Robo-Signalweg | de |
| dc.contributor.referee | Vorbrüggen, Gerd Dr. | de |
| dc.date.examination | 2007-11-06 | de |
| dc.subject.dnb | 500 Naturwissenschaften | de |
| dc.subject.gok | WKF 300 | de |
| dc.description.abstracteng | Syndecan (Sdc) is a type I transmembrane
protein characterized by the presence of linear sugar chains called
heparan sulphate glycosaminoglycans (HSGAGs) linked to its
extracellular domain. Sdc was shown to be critical for the fidelity
of Slit repellent signalling at the Drosophila midline where via
its receptor Robo, Slit patterns the central nervous system (CNS)
and the musculature. This study aimed at shedding some light on how
Sdc functions in Slit/Robo signalling. Employing the UAS/GAL4
system, different sdc variants and chimeric transgenes were
expressed in specific tissues in a sdc mutant background and tested
for their rescue capability. Rescue was scored by the absence of
ventral midline crossing of ipsilateral axons and ventral muscles.
The in vivo rescue experiments proved that Drosophila Sdc is
specifically required on the target tissue where it functions as a
coreceptor with Robo and that Sdc has no function in Slit secretion
or transport nor does it have an independent intracellular
signalling activity. Furthermore, the results also support a model
in which shedding of Sdc though not required for the fidelity of
Slit repellent signalling could occur by the proteolytic activity
of a serine protease which might function as a negative feedback
system or might be required for the recycling of Robo
receptor. | de |
| dc.contributor.coReferee | Wimmer, Ernst A. Prof. Dr. | de |
| dc.contributor.thirdReferee | Pieler, Tomas Prof. Dr. | de |
| dc.subject.topic | Mathematics and Natural Science | de |
| dc.subject.ger | Syndecan | de |
| dc.subject.ger | Heparansulfatproteoglykan | de |
| dc.subject.ger | Slit | de |
| dc.subject.ger | Robo | de |
| dc.subject.ger | Axone | de |
| dc.subject.eng | Syndecan | de |
| dc.subject.eng | heparan sulphate proteoglycan | de |
| dc.subject.eng | Slit | de |
| dc.subject.eng | Robo | de |
| dc.subject.eng | axon guidance | de |
| dc.subject.bk | 42.23 | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-1770-5 | de |
| dc.identifier.purl | webdoc-1770 | de |
| dc.identifier.ppn | 588951625 | de |