A Systems Level Analysis of Neuronal Network Function in the Olfactory Bulb: Coding, Connectivity, and Modular organization

Chen, Tsai-Wen

dc.contributor.advisor	Schild, Detlev Prof. Dr. Dr.	de
dc.contributor.author	Chen, Tsai-Wen	de
dc.date.accessioned	2013-01-22T15:47:52Z	de
dc.date.available	2013-01-30T23:50:59Z	de
dc.date.issued	2008-12-15	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-F166-0	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3468
dc.format.mimetype	application/pdf	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	A Systems Level Analysis of Neuronal Network Function in the Olfactory Bulb: Coding, Connectivity, and Modular organization	de
dc.type	doctoralThesis	de
dc.title.translated	A Systems Level Analysis of Neuronal Network Function in the Olfactory Bulb: Coding, Connectivity, and Modular organization	de
dc.contributor.referee	Wolf, Fred Prof. Dr.	de
dc.date.examination	2008-05-08	de
dc.subject.dnb	570 Biowissenschaften, Biologie	de
dc.subject.gok	WCK 000	de
dc.subject.gok	WA 310	de
dc.subject.gok	MED 311	de
dc.description.abstracteng	The olfactory bulb (OB) is the first brain region that receives and processes sensory inputs from the nose. It contains highly specialized neuronal networks organized by individual glomeruli, which are sites of synaptic contacts between olfactory sensory neurons (OSN) and secondary mitral/tufted (M/T) cells in the OB. In this thesis, I explored the neuronal network function of a vertebrate OB system, using a combination of calcium imaging and electrophysiological techniques. In the first part of the thesis, I introduced a number of image processing techniques for analyzing functional calcium imaging data. This facilitates the selection of regions of interest and allows visualizing fine processes of neurons in densely stained brain tissues. In the second part of the thesis, we explored the dynamics of [Ca2+] activity of M/T cell population. This led to the identification of distinct subpopulations of neurons that showed highly synchronous spontaneous [Ca2+] activity. Anatomical reconstruction revealed that these synchronous neurons were invariably connected to the same glomerulus. Furthermore, the odor-induced responses of synchronous neurons were virtually identical, irrespective of whether the responses were excitatory or inhibitory, and irrespective of the distance between them. In the third part of the thesis, we analyzed the mechanism underlying the synchronous responses. We show that synchronous neurons received correlated EPSC inputs and were coupled by gap junctions. Together, these data reveal that odors are represented in the OB by modules of distributed and synchronous M/T cells associated with the same glomeruli. This may allow for parallel input to higher brain centers.	de
dc.contributor.coReferee	Stühmer, Walter Prof. Dr.	de
dc.subject.topic	Molecular Biology & Neurosciences Program	de
dc.subject.ger	Calcium imaging	de
dc.subject.ger	Olfactory bulb	de
dc.subject.ger	Mitral Cell	de
dc.subject.ger	Synchronous activity	de
dc.subject.ger	Glomerulus	de
dc.subject.ger	Neuronal Network	de
dc.subject.ger	Image Processing	de
dc.subject.eng	Calcium imaging	de
dc.subject.eng	Olfactory bulb	de
dc.subject.eng	Mitral Cell	de
dc.subject.eng	Synchronous activity	de
dc.subject.eng	Glomerulus	de
dc.subject.eng	Neuronal Network	de
dc.subject.eng	Image Processing	de
dc.subject.bk	42.17	de
dc.subject.bk	42.12	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-1794-3	de
dc.identifier.purl	webdoc-1794	de
dc.identifier.ppn	596076037	de

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