dc.contributor.advisor | Stülke, Jörg Prof. Dr. | de |
dc.contributor.author | Lehnik-Habrink, Martin | de |
dc.date.accessioned | 2013-01-30T11:32:44Z | de |
dc.date.available | 2013-01-30T23:51:29Z | de |
dc.date.issued | 2011-12-14 | de |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-000D-F1E1-7 | de |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-3554 | |
dc.description.abstract | Der Abbau von mRNA Molekülen ist ein
fundamentaler Prozess aller Lebewesen. Dieser Schritt dient nicht
nur der Rückgewinnung von Nukleotiden, sondern reguliert auch die
Genexpression durch die Verfügbarkeit von mRNA Matrizen. Die
wichtigsten Spieler in diesem Prozess sind RNasen. Diese können als
einzelne Enzyme arbeiten oder in Multiproteinkomplexen vorkommen,
den sogenannten RNA-Degradosomen. Ein solches RNA Degradosom wurde
kürzlich auch für den Gram-positiven Modelorganismus Bacillus
subtilis beschrieben. Die Untersuchung dieses Komplexes und
detailierte Analysen der beteiligten Enzyme waren die Ziele dieser
Arbeit. | de |
dc.format.mimetype | application/pdf | de |
dc.language.iso | eng | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | de |
dc.title | An mRNA degradation complex in Bacillus subtilis | de |
dc.type | doctoralThesis | de |
dc.title.translated | mRNA Abbau in Bacillus subtilis | de |
dc.contributor.referee | Stülke, Jörg Prof. Dr. | de |
dc.date.examination | 2011-10-26 | de |
dc.subject.dnb | 570 Biowissenschaften, Biologie | de |
dc.subject.gok | WF 200 | de |
dc.description.abstracteng | In every organism the degradation of mRNAs
is a fundamental process. This step not only recycles nucleotides
but also adjusts gene expression due to the availability of the
mRNA templates. The main players in this process are RNases which
can function as single enzymes or in multiprotein complexes, the
so-called RNA degradosomes. Recently, the existence such an RNA
degradosome was demonstrated in the Gram-positive model organism
Bacillus subtilis. The investigation of the complex and detailed
analyses of some of its members was the aim of this work. The
initial model of the RNA degradosome of B. subtilis consists of
RNases and glycolytic enzymes. The central RNase of the complex is
the endoribonuclease RNase Y. To assess the impact of RNase Y on
mRNA decay we performed microarray analysis demonstrating that
depletion of the enzyme affects the degradation of transcripts on
global scale. Further analysis of several targets revealed that
RNase Y is indeed crucial for the turnover of the transcripts
resulting in remarkably increased half lives upon depletion of the
enzyme. These experiments highlight RNase Y as a key player in the
mRNA degradation of B. subtilis. Due to its high significance in
the mRNA decay process we further investigated RNase Y for its
physiological and biochemical properties. We studied its domain
organization and revealed that the protein contains substantial
regions of intrinsic disorder. Furthermore we demonstrated that the
membrane localization of RNase Y is essential for survival. As
mentioned, the initial model of the RNA degradosome contained only
RNases and glycolytic enzymes. In contrast to homolog complexes in
other bacteria, a DEAD-box RNA helicase was not present in our
model. Therefore we re-addressed this issue and revealed that the
DEAD-box RNA helicase CshA has the ability to interact with all
enzymes of the complex, especially with the important RNase Y. The
suggested participation of CshA in the RNA degrading complex is
underlined by the fact that deletion of cshA affects the abundance
of hundreds of mRNAs. In addition to its participation in the RNA
degradosome, we found that CshA is implicated in the biogenesis of
ribosomes and the adaption to low temperatures. In conclusion, the
analysis of RNase Y and CshA as members of the RNA degradosome
revealed the impact of both enzymes for the physiology of B.
subtilis and pave the way for further investigations. | de |
dc.contributor.coReferee | Tittmann, Kai Prof. Dr. | de |
dc.contributor.thirdReferee | Kramer, Wilfried PD Dr. | de |
dc.subject.topic | Biology (incl. Psychology) | de |
dc.subject.ger | Bacillus subtilis | de |
dc.subject.ger | RNA Abbau | de |
dc.subject.ger | RNase | de |
dc.subject.ger | mRNA | de |
dc.subject.ger | RNA Helikase | de |
dc.subject.ger | Degradosom | de |
dc.subject.ger | Proteinkomplex | de |
dc.subject.ger | DEAD-box | de |
dc.subject.eng | Bacillus subtilis | de |
dc.subject.eng | RNA degradation | de |
dc.subject.eng | RNase | de |
dc.subject.eng | mRNA | de |
dc.subject.eng | RNA helicase | de |
dc.subject.eng | degradosome | de |
dc.subject.eng | proteincomplex | de |
dc.subject.eng | DEAD-box | de |
dc.subject.bk | 42 Biologie | de |
dc.identifier.urn | urn:nbn:de:gbv:7-webdoc-3286-8 | de |
dc.identifier.purl | webdoc-3286 | de |
dc.affiliation.institute | Biologische Fakultät | de |
dc.identifier.ppn | 730113353 | de |