An mRNA degradation complex in Bacillus subtilis

Lehnik-Habrink, Martin

dc.contributor.advisor	Stülke, Jörg Prof. Dr.	de
dc.contributor.author	Lehnik-Habrink, Martin	de
dc.date.accessioned	2013-01-30T11:32:44Z	de
dc.date.available	2013-01-30T23:51:29Z	de
dc.date.issued	2011-12-14	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-F1E1-7	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3554
dc.description.abstract	Der Abbau von mRNA Molekülen ist ein fundamentaler Prozess aller Lebewesen. Dieser Schritt dient nicht nur der Rückgewinnung von Nukleotiden, sondern reguliert auch die Genexpression durch die Verfügbarkeit von mRNA Matrizen. Die wichtigsten Spieler in diesem Prozess sind RNasen. Diese können als einzelne Enzyme arbeiten oder in Multiproteinkomplexen vorkommen, den sogenannten RNA-Degradosomen. Ein solches RNA Degradosom wurde kürzlich auch für den Gram-positiven Modelorganismus Bacillus subtilis beschrieben. Die Untersuchung dieses Komplexes und detailierte Analysen der beteiligten Enzyme waren die Ziele dieser Arbeit.	de
dc.format.mimetype	application/pdf	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	An mRNA degradation complex in Bacillus subtilis	de
dc.type	doctoralThesis	de
dc.title.translated	mRNA Abbau in Bacillus subtilis	de
dc.contributor.referee	Stülke, Jörg Prof. Dr.	de
dc.date.examination	2011-10-26	de
dc.subject.dnb	570 Biowissenschaften, Biologie	de
dc.subject.gok	WF 200	de
dc.description.abstracteng	In every organism the degradation of mRNAs is a fundamental process. This step not only recycles nucleotides but also adjusts gene expression due to the availability of the mRNA templates. The main players in this process are RNases which can function as single enzymes or in multiprotein complexes, the so-called RNA degradosomes. Recently, the existence such an RNA degradosome was demonstrated in the Gram-positive model organism Bacillus subtilis. The investigation of the complex and detailed analyses of some of its members was the aim of this work. The initial model of the RNA degradosome of B. subtilis consists of RNases and glycolytic enzymes. The central RNase of the complex is the endoribonuclease RNase Y. To assess the impact of RNase Y on mRNA decay we performed microarray analysis demonstrating that depletion of the enzyme affects the degradation of transcripts on global scale. Further analysis of several targets revealed that RNase Y is indeed crucial for the turnover of the transcripts resulting in remarkably increased half lives upon depletion of the enzyme. These experiments highlight RNase Y as a key player in the mRNA degradation of B. subtilis. Due to its high significance in the mRNA decay process we further investigated RNase Y for its physiological and biochemical properties. We studied its domain organization and revealed that the protein contains substantial regions of intrinsic disorder. Furthermore we demonstrated that the membrane localization of RNase Y is essential for survival. As mentioned, the initial model of the RNA degradosome contained only RNases and glycolytic enzymes. In contrast to homolog complexes in other bacteria, a DEAD-box RNA helicase was not present in our model. Therefore we re-addressed this issue and revealed that the DEAD-box RNA helicase CshA has the ability to interact with all enzymes of the complex, especially with the important RNase Y. The suggested participation of CshA in the RNA degrading complex is underlined by the fact that deletion of cshA affects the abundance of hundreds of mRNAs. In addition to its participation in the RNA degradosome, we found that CshA is implicated in the biogenesis of ribosomes and the adaption to low temperatures. In conclusion, the analysis of RNase Y and CshA as members of the RNA degradosome revealed the impact of both enzymes for the physiology of B. subtilis and pave the way for further investigations.	de
dc.contributor.coReferee	Tittmann, Kai Prof. Dr.	de
dc.contributor.thirdReferee	Kramer, Wilfried PD Dr.	de
dc.subject.topic	Biology (incl. Psychology)	de
dc.subject.ger	Bacillus subtilis	de
dc.subject.ger	RNA Abbau	de
dc.subject.ger	RNase	de
dc.subject.ger	mRNA	de
dc.subject.ger	RNA Helikase	de
dc.subject.ger	Degradosom	de
dc.subject.ger	Proteinkomplex	de
dc.subject.ger	DEAD-box	de
dc.subject.eng	Bacillus subtilis	de
dc.subject.eng	RNA degradation	de
dc.subject.eng	RNase	de
dc.subject.eng	mRNA	de
dc.subject.eng	RNA helicase	de
dc.subject.eng	degradosome	de
dc.subject.eng	proteincomplex	de
dc.subject.eng	DEAD-box	de
dc.subject.bk	42 Biologie	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3286-8	de
dc.identifier.purl	webdoc-3286	de
dc.affiliation.institute	Biologische Fakultät	de
dc.identifier.ppn	730113353	de

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