Interaktion des hNaDC3 mit Fumarat und Fumaratderivaten

Schmidt, Andrea Isabella

dc.contributor.advisor	Burckhardt, Birgitta-Christina Prof. Dr.	de
dc.contributor.author	Schmidt, Andrea Isabella	de
dc.date.accessioned	2013-04-22T08:18:21Z	de
dc.date.available	2013-05-06T22:50:05Z	de
dc.date.issued	2013-04-22	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0015-9BFF-1	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3805
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Interaktion des hNaDC3 mit Fumarat und Fumaratderivaten	de
dc.type	doctoralThesis	de
dc.title.translated	hNaDC3 and its interactions with fumararate and fumarate derivates	de
dc.contributor.referee	Mößner, Rotraut PD Dr.	de
dc.date.examination	2013-04-29	de
dc.description.abstracteng	Human NaDC3 is one of the five members of the “solute carrier 13 (SLC13)” gene family, all of them coding for electrogenic sodium-coupled transporters on plasma membranes. Transcripts of hNaDC3 have been detected in renal, hepatic, cerebral and placenta tissue. In a sodium-dependent symport it carries different dicarboxylates like succinate across the plasma membrane into the cytosol. Because of the increasing clinical relevance of fumarate and its derivates, we analysed the transport properties of hNaDC3 concerning these substrates, especially in comparison to succinate, a substrate of hNaDC3. First, we measured uptake of radiolabeled succinate in presence of sodium into hNaDC3- and vector-transfected HEK293 cells, adding fumarate, dimethylfumarate and diethylfumarate sequential to the extracellular solution. We only noticed inhibition of succinate uptake in presence of fumarate, showing affinity of hNaDC3 to fumarate, but not to diethylfumarate and dimethylfumarate. Second, we performed experiments using a two electrode voltage clamp device, measuring membrane currents in dependence of the substrates named above. Inward currents were could be identified under varied membrane potentials for the substrates succinate and fumarate. Again, there was no sign of transport activity noticeable for diethylfumarate and dimethylfumarate.	de
dc.contributor.coReferee	Virsik-Köpp, Patricia Prof. Dr.	de
dc.subject.eng	hNaDC3, solute carrier, fumarate, diethylfumarate, dimethylfumarate	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0015-9BFF-1-8	de
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Physiologie / Pathophysiologie - Allgemein- und Gesamtdarstellungen (PPN619875283)	de
dc.description.embargoed	2013-05-06	de
dc.identifier.ppn	746434413	de

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Human- und Zahnmedizin [2872]

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