Biophysics of Blood Platelet Contraction

Schwarz G. Henriques, Sarah

dc.contributor.advisor	Köster, Sarah Prof. Dr.	de
dc.contributor.author	Schwarz G. Henriques, Sarah	de
dc.date.accessioned	2013-05-21T09:16:19Z	de
dc.date.available	2013-05-21T09:16:19Z	de
dc.date.issued	2013-05-21	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-001C-21C5-C	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-3853
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	530	de
dc.title	Biophysics of Blood Platelet Contraction	de
dc.type	doctoralThesis	de
dc.contributor.referee	Köster, Sarah Prof. Dr.	de
dc.date.examination	2012-07-10	de
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	This work is part of a project on blood platelet contraction using an innovative double approach to experiments. First, we adapted the experimental technique, traction force microscopy (TFM), to our cellular system, and measured forces generated by single, activated platelets with hitherto unknown spatial and temporal resolution. In a second set of experiments, we looked at both force generators and force transduction sites (focal adhesions) in platelets that were activated on soft polyacrylamide (PAA) substrates, used in kind already for TFM. Here, we fixed platelets and stained platelet components during different stages of activation. By combining these two experimental approaches, we were able to establish connections between characteristics of measured force fields and the (re-)organization of both the platelet cytoskeleton and focal adhesion sites. Our findings have several implications for experimental concepts applied to future force measurements of platelet contraction. In addition, our experiments on PAA have revealed that platelet activation on materials with physiological elasticities proceeds in a slightly different manner than on glass, the traditional choice of substrate. Lastly, our measurements of local forces illustrate specific characteristics of platelet force fields, which will affect future mechanical models of platelet contraction.	de
dc.contributor.coReferee	Herminghaus, Stephan Prof. Dr.	de
dc.subject.eng	focal adhesions;	de
dc.subject.eng	blood platelets;	de
dc.subject.eng	cytoskeletal reorganization;	de
dc.subject.eng	traction forces;	de
dc.subject.eng	force fields;	de
dc.subject.eng	cellular contraction;	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-001C-21C5-C-5	de
dc.affiliation.institute	Fakultät für Physik	de
dc.identifier.ppn	749454695	de

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Fakultät für Physik (inkl. GAUSS) [925]

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