Regulation der „spleen tyrosine kinase“ Syk im B-Zell-Antigen-Rezeptor-Signalweg

Bohnenberger, Hanibal

dc.contributor.advisor	Wienands, Jürgen Prof. Dr.
dc.contributor.author	Bohnenberger, Hanibal
dc.date.accessioned	2013-11-25T08:27:33Z
dc.date.available	2014-01-21T23:50:05Z
dc.date.issued	2013-11-25
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-001D-BFEC-7
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4170
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Regulation der „spleen tyrosine kinase“ Syk im B-Zell-Antigen-Rezeptor-Signalweg	de
dc.type	doctoralThesis	de
dc.title.translated	Regulation of the "spleen tyrosine kinase" Syk in the B-cell antigen receptor signaling pathway	de
dc.contributor.referee	Wienands, Jürgen Prof. Dr.
dc.date.examination	2014-01-14
dc.description.abstracteng	The “spleen tyrosine kinase” Syk offers transducer functions for a number of ITAM-based immune cell receptors and is critical for the development and activation of B lymphocytes. Furthermore Syk has been implicated in the pathogenesis of several forms of leukemia. Its catalytic activity and the capacity to interact with other signaling elements depend on its phosphorylation status. In this thesis the full spectrum of phosphoacceptor sites in human Syk and its complex dynamics in response to B-Cell antigen receptor stimulation was determined by high resolution mass spectrometry. One of the most frequently detected phosphoacceptor sites was serine 297. By elucidating the Syk interactome in resting and activated B cells, consisting of more than 25 interacting proteins, the 14-3-3 family of adapter proteins was discovered as one new group of interacting partners, which bind directly to phospho serine 297 after BCR stimulation. The latter complex weakens inducible plasma membrane recruitment of Syk and inhibits the B-cell antigen receptor signaling pathway. Collectively, the established ligand and phosphoacceptor site library offers a basis to comprehend the complexity of the Syk signaling network. The described regulation of Syk by 14-3-3 is the first reported serine dependent inhibition of Syk.	de
dc.contributor.coReferee	Pieler, Tomas Prof. Dr.
dc.subject.ger	B Zell Antigen Rezeptor	de
dc.subject.ger	Massenspektrometrie	de
dc.subject.ger	Proteomik	de
dc.subject.eng	B cell antigen receptor	de
dc.subject.eng	SILAC	de
dc.subject.eng	Mass spectrometry	de
dc.subject.eng	Syk	de
dc.subject.eng	Proteomics	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-001D-BFEC-7-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Immunologie	de
dc.subject.gokfull	Allergologie	de
dc.subject.gokfull	Umweltmedizin	de
dc.subject.gokfull	Medizinische Ökologie Allgemein- und Gesamtdarstellungen	de
dc.description.embargoed	2014-01-21
dc.identifier.ppn	772201919

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