Einfluss des extrazellulären pH-Werts auf den Transport von para-Aminohippurat über den organischen Anionentransporter 1

Engelke, Christian

dc.contributor.advisor	Burckhardt, Birgitta-Christina Prof. Dr.
dc.contributor.author	Engelke, Christian
dc.date.accessioned	2015-02-10T09:21:07Z
dc.date.available	2015-02-23T23:50:05Z
dc.date.issued	2015-02-10
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5DB1-7
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4899
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Einfluss des extrazellulären pH-Werts auf den Transport von para-Aminohippurat über den organischen Anionentransporter 1	de
dc.type	doctoralThesis	de
dc.title.translated	Influence of the extracellular pH on the transport of para-aminohippurate via organic anion transporter 1	de
dc.contributor.referee	Burckhardt, Birgitta-C. Prof. Dr.
dc.date.examination	2015-02-16
dc.description.abstracteng	Organic anion transporter 1 (OAT1) is one of the transporters responsible for renal elimination of organic anions. In the present study, the influence of extracellular pH on the transport-activity of OAT1 was examined using OAT1-transfected HEK 293 cells and the model substrate para-aminohippurate (PAH). The uptake of [3H]PAH was measured by a liquid scintillation counter at different time points, varying extracellular pH and chemicals possibly influencing pH-dependent processes. In general, uptake of PAH was higher at lower than at higher extracellular pH. Probenecid fully inhibited PAH uptake regardless of the extracellular pH. At pH 6.4 and pH 7.4, Michaelis-Menten constant remained the same, but Vmax increased. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) inhibited the uptake of PAH with an IC50 of 2,5 ± 0,5 µM. The pH dependence on the uptake of PAH remained in the presence of CCCP. Hydrogen carbonate has no influence on the uptake of PAH whereas acetazolamide, 4-acetamido-4-isothiocyanostilbene-2,2-disulfonate (SITS) and amiloride inhibited the uptake of PAH at pH 6.4 and 7.4.	de
dc.contributor.coReferee	Kehlenbach, Ralph Prof. Dr.
dc.subject.eng	oat1	de
dc.subject.eng	hoat1	de
dc.subject.eng	ph	de
dc.subject.eng	human organic anion transporter 1	de
dc.subject.eng	organic anion transporter 1	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5DB1-7-7
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.description.embargoed	2015-02-23
dc.identifier.ppn	817857974

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Human- und Zahnmedizin [2872]

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