Wirkung von zwei verschieden konzentrierten alkoholfreien CHX-Mundspüllösungen auf die Regenerationsfähigkeit von humanen Gingivafibroblasten

eine In-vitro-Studie

Effect of two different concentrations alcohol-free CHX mouthrinses on the regeneration ability of human gingival fibroblasts

an in vitro study

by Natalja Schalaikin
Doctoral thesis
Date of Examination:2014-02-25
Date of issue:2014-02-17
Advisor:Prof. Dr. Rainer Mausberg
Referee:PD Dr. Holger Hänßle
Referee:Prof. Dr. Elisabeth Zeisberg
Persistent Address: http://dx.doi.org/10.53846/goediss-4366

 

 

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Abstract

English

Increasing importance is given to the antiseptic, anti-inflammatory and tooth protec-tion properties of chlorhexidine mouth washes, as the mechanical plaque reduction is often insufficient. Numerous studies have proven and demonstrated the prophy-lactic and therapeutical effects of mouth washes, none have however systematically examined their compatibility. As these products are frequently used over a period of many years, knowledge of the tissue or histo compatibility is very important. This study aims to examine the influence that CHX mouth washes (using two com-mercial concentrations) have on human gingiva fibroblasts and to investigate possi-ble in vitro revitalization. For this purpose, frozen gingiva fibre blast cells from a hea-lthy periodontium female proband were used and cultivated up to the fifth passage. The cultivation was carried out at 37° degrees Celsius and 5% CO2. The cells were exposed to 0,12% and 0,2% CHX for one and five minutes and then compared to the untreated control cells according to certain parameters. The cell viability was investigated during the experimental set-up using an MTT-assay and Cellometer. The cell number was examined using the Cellometer during three de-fined time intervals (24 hours, three days, and five days). These tests were repeated twelve times. The test results showed that both CHX mouth wash concentrations influenced the gingiva fibroblast cells significantly: they seemed to be cytotoxic. The cytotoxicity presented itself dose- and time-dependent. Although the cell viability had increased on the fifth day after an initial strong decline, it was still significantly lower than the viability of the control cells. The results of the present study confirm other relevant publications as to the cytotoxic effects of Chlorhexin on gingiva fibroblast cells. Cu-mulatively all results show that more than one parameter is needed to assess the cell functions and to analyze the effects that CHX has on the fibre blasts in vitro. By merely taking the cell number, viability and size into consideration, as was done in previous studies, one does not get a full picture as to the effectivity of the CHX sub-stance. They do however help for further clarification. Nonetheless, the process of obtaining new insights and information is not complete. To date only a few In-vitro studies have been conducted using this type of human cell, with realistic exposure times and different CHX concentrations, thus allowing for the derivation of regenera-tion times and mechanisms. Many questions remain unanswered. Due to these cytotoxic effects of chlorhexidine on gingiva fibre blast cells, a prophy-lactic or preventive treatment of a healthy periodontal condition should be clearly questioned and only undertaken if strict indications warrant it. Based on the results of this study, the direct rinsing or flushing of exposed periodontal bone or connective tissue with CHX solutions during a periodontal surgical measure should strongly be reconsidered. On the other hand, the fact must be considered, that the healing pro-cess will more likely be disturbed by bacterial infections than by the careful applica-tion of this antiseptic treatment. It is recommended that further toxicological and cy-totoxical analyses as to the effects of CHX mouth washes are conducted to ensure the safety of these commercially available products and assure a focused and effec-tive application in dental medicine.
Keywords: CHX mouth washes+regeneration+gingiva fibroblasts+toxication+effect
Schlagwörter: Chlorhexidinmundspüllösung+Regeneration +humane Gingivafibroblasten
 
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