dc.contributor.advisor | Miosge, Nicolai Prof. Dr. | |
dc.contributor.author | Klein, Moritz | |
dc.date.accessioned | 2014-03-26T09:13:34Z | |
dc.date.available | 2014-04-02T22:50:05Z | |
dc.date.issued | 2014-03-26 | |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0022-5E6C-0 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4428 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4428 | |
dc.language.iso | deu | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject.ddc | 610 | de |
dc.title | Knockdown von Runx2 durch RNA-Interference in chondrogenen Progenitor-Zellen | de |
dc.type | doctoralThesis | de |
dc.title.translated | Knockdown of Runx2 with RNA-interference in chondrogenic progenitor cells | de |
dc.contributor.referee | Miosge, Nicolai Prof. Dr. | |
dc.date.examination | 2014-03-26 | |
dc.description.abstracteng | The regeneration of diseased hyaline cartilage continues to be a great challenge because its self renewal capacity is limited.
In repair tissue from human articular cartilage during the late stages of osteoarthritis chondrogenic progenitor cells (CPC`s) were found. These cells showed stemm cell capacity such as multipotency and migratory activity.
The Transkriptionfaktor Runt-related transkription Factor 2 (Runx2) is an importend regulator of Osteogenesis and is also expressed in CPC`s.
We tried to generate a Knockdown of Runx2 in CPC`S with the help of an lentiviral Vektor and RNA-Interference. We also investigated the influence of Runx2 knockdown to the chondrogenic transkriptionfactor SRY-related HMG-box gene 9 (Sox9).
Our results showed that the transfection of CPC`s with the i-lenti-GFP expression Vector (Biocat) with Amaxa Nukleofection leads to transient transfektion. The Knockdown of Runx2 could be showed to 96 h after transfektion with Polymerase chain reaction (RT-PCR) and to 48 h in the western blot.
We also could show a changing in sox9 expression in the transfected CPC`s. | de |
dc.contributor.coReferee | Wiese, Karl Günter Prof. Dr. Dr. | |
dc.subject.eng | Runx2 | de |
dc.subject.eng | Sox9 | de |
dc.subject.eng | Osteoarthritis | de |
dc.subject.eng | RNA-interference | de |
dc.subject.eng | chondrogenic progenitorcells | de |
dc.subject.eng | Nucleofaction | de |
dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5E6C-0-3 | |
dc.affiliation.institute | Medizinische Fakultät | de |
dc.subject.gokfull | Medizin (PPN619874732) | de |
dc.subject.gokfull | Labormedizin / Klinische Chemie - Allgemein- und Gesamtdarstellungen (PPN61987564X) | de |
dc.description.embargoed | 2014-04-02 | |
dc.identifier.ppn | 781600987 | |