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Knockdown von Runx2 durch RNA-Interference in chondrogenen Progenitor-Zellen

dc.contributor.advisorMiosge, Nicolai Prof. Dr.
dc.contributor.authorKlein, Moritz
dc.date.accessioned2014-03-26T09:13:34Z
dc.date.available2014-04-02T22:50:05Z
dc.date.issued2014-03-26
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-0022-5E6C-0
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-4428
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-4428
dc.language.isodeude
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc610de
dc.titleKnockdown von Runx2 durch RNA-Interference in chondrogenen Progenitor-Zellende
dc.typedoctoralThesisde
dc.title.translatedKnockdown of Runx2 with RNA-interference in chondrogenic progenitor cellsde
dc.contributor.refereeMiosge, Nicolai Prof. Dr.
dc.date.examination2014-03-26
dc.description.abstractengThe regeneration of diseased hyaline cartilage continues to be a great challenge because its self renewal capacity is limited.  In repair tissue from human articular cartilage during the late stages of osteoarthritis chondrogenic progenitor cells (CPC`s) were found. These cells showed stemm cell capacity such as multipotency and migratory activity. The Transkriptionfaktor Runt-related transkription Factor 2 (Runx2) is an importend regulator of Osteogenesis and is also expressed in CPC`s.  We tried to generate a Knockdown of Runx2 in CPC`S with the help of an lentiviral Vektor and RNA-Interference. We also investigated the influence of Runx2 knockdown to the chondrogenic transkriptionfactor SRY-related HMG-box gene 9 (Sox9). Our results showed that the transfection of CPC`s with the i-lenti-GFP expression Vector (Biocat) with Amaxa Nukleofection leads to transient transfektion. The Knockdown of Runx2 could be showed to 96 h after transfektion with Polymerase chain  reaction (RT-PCR) and to 48 h in the western blot. We also could show a changing in sox9 expression in the transfected CPC`s.de
dc.contributor.coRefereeWiese, Karl Günter Prof. Dr. Dr.
dc.subject.engRunx2de
dc.subject.engSox9de
dc.subject.engOsteoarthritisde
dc.subject.engRNA-interferencede
dc.subject.engchondrogenic progenitorcellsde
dc.subject.engNucleofactionde
dc.identifier.urnurn:nbn:de:gbv:7-11858/00-1735-0000-0022-5E6C-0-3
dc.affiliation.instituteMedizinische Fakultätde
dc.subject.gokfullMedizin (PPN619874732)de
dc.subject.gokfullLabormedizin / Klinische Chemie - Allgemein- und Gesamtdarstellungen (PPN61987564X)de
dc.description.embargoed2014-04-02
dc.identifier.ppn781600987


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