Investigation of protein-RNA interactions by UV cross-linking and mass spectrometry: methodological improvements toward in vivo applications

Kramer, Katharina

dc.contributor.advisor	Urlaub, Henning Prof. Dr.
dc.contributor.author	Kramer, Katharina
dc.date.accessioned	2014-04-07T09:48:17Z
dc.date.available	2014-04-07T09:48:17Z
dc.date.issued	2014-04-07
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5E7C-C
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4445
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4445
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4445
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	572	de
dc.title	Investigation of protein-RNA interactions by UV cross-linking and mass spectrometry: methodological improvements toward in vivo applications	de
dc.type	doctoralThesis	de
dc.contributor.referee	Urlaub, Henning Prof. Dr.
dc.date.examination	2013-05-30
dc.description.abstracteng	Protein–RNA complexes play key roles in a variety of cellular functions such as gene expression and its regulation. One frequently applied method for the investigation of direct protein–RNA interactions is UV induced cross-linking. Mass spectrometry (MS) is increasingly utilized for the identification of proteins and peptides directly interacting with RNA. The major challenges for this application are the generally low yield of the UV induced cross-linking reaction and the lack of suitable tools for MS data analysis. In this work, both issues are adressed. A novel cross-linking pathway involving net loss of H2S from the photoreactive base-analogue 4-thio-uracil was identified, and a novel approach for automated identification of cross-linked peptides from mass spectrometry data was developed.	de
dc.contributor.coReferee	Stülke, Jörg Prof. Dr.
dc.subject.eng	proteomics	de
dc.subject.eng	protein-RNA interactions	de
dc.subject.eng	UV cross-linking	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5E7C-C-4
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	782477240

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Das Dokument erscheint in:

GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1115]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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