Der Einfluss unterschiedlicher Zellkulturmedien auf die Makrophagen in einem Co-Kultur-Modell von Nervengewebe und Peritonealzellen

Schulte, Jana

dc.contributor.advisor	Brück, Wolfgang Prof. Dr.
dc.contributor.author	Schulte, Jana
dc.date.accessioned	2014-05-08T08:47:24Z
dc.date.available	2014-05-20T22:50:03Z
dc.date.issued	2014-05-08
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5EA8-6
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4480
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4480
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4480
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Der Einfluss unterschiedlicher Zellkulturmedien auf die Makrophagen in einem Co-Kultur-Modell von Nervengewebe und Peritonealzellen	de
dc.type	doctoralThesis	de
dc.title.translated	The differential influence of cell culture media on macrophages in a co-culture model of nerve tissue and peritoneal cells.	de
dc.contributor.referee	Reichardt, Holger Prof. Dr.
dc.date.examination	2014-05-13
dc.description.abstracteng	During Wallerian degeneration in the central or peripheral nervous system the migration of macrophages to the injured tissue can be observed. Then these cells phagocytize the accruing myelin and axon debris at the injury site. In a co-culture model of mouse sciatic nerves and peritoneal macrophages we examined the phagocytic capacity of macrophages under different culture conditions. Small pieces of sciatic nerve remained for 8 days in culture together with thioglycollate-elicited macrophages either in DMEM + 10% FCS or in serumfree Iscove's MEM (Panserin) supplemented with N2 components. We found that macrophages developed differentially in the two culture media. Macrophages in serum-free medium were larger in the intial phase of culturing, but later they showed morphological differences with regard to the appearance of fat vacuoles within the cells. These findings were confirmed by a special culturing method in "ibidi"-3D culture dishes where in which the cells were exposed to both media. Macrophages in the Panserin compartment were larger and cell survival was much prolonged in Panserin in contrast to DMEM+FCS. Immunohistochemistry for different macrophage markers revealed the highest expressions for Mac-3, Mac-1, CD68, F4/80 and scavenger receptor CD204. In DMEM+FCS all expression levels were higher than in Panserin, suggesting that DMEM+FCS has a higher potential of immunomodulation – probably due to the content of FCS. The amount of fat vacuoles within the cells was evaluated by ORO staining and revealing slightly lower numbers but larger sizes in DMEM+FCS in contrast to Panserin (higher numbers of lower size), which indicated a differentially regulated myelin uptake within the macrophages and an altered myelin processing in these different media. By overlaying photomicrographs of ORO-stainings with different macrophage markers several double stainings could be shown, but also single stainings for either a macrophage marker or for fat vacuoles are visible. This indicates that not all positive cells contain fat vacuoles or vice versa, and that not all fat vacuoles have been processed by macrophages. Our results indicate that macrophages are able to react in the presence or absence of serum in culture media. The different culturing conditions and in particular FCS obviously influence immunocompetence and cellular catabolism in macrophages as well as their migratory potential. These findings might afford insights in the phagocytic capacity of macrophages and their cellular differentiation.	de
dc.contributor.coReferee	Oppermann, Martin Prof. Dr.
dc.subject.eng	macrophages	de
dc.subject.eng	Wallerian Degeneration	de
dc.subject.eng	different culturing conditions	de
dc.subject.eng	co-culture model of mouse sciatic nerves	de
dc.subject.eng	Panserin	de
dc.subject.eng	DMEM+FCS	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5EA8-6-9
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.subject.gokfull	Neuroanatomie, Neurophysiologie, Neuropathologie (PPN619876255)	de
dc.description.embargoed	2014-05-20
dc.identifier.ppn	785242910

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Human- und Zahnmedizin [2873]

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