Nucleosylaminosäuren als Bausteine zur Synthese modifizierter Oligonucleotide

Schmidtgall, Boris

dc.contributor.advisor	Ducho, Christian Prof. Dr.
dc.contributor.author	Schmidtgall, Boris
dc.date.accessioned	2014-05-13T09:28:02Z
dc.date.available	2014-05-13T09:28:02Z
dc.date.issued	2014-05-13
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5EB1-0
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4502
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	571.4	de
dc.title	Nucleosylaminosäuren als Bausteine zur Synthese modifizierter Oligonucleotide	de
dc.type	doctoralThesis	de
dc.title.translated	Nucleosyl Amino Acids as Building Blocks for the Synthesis of Modified Oligonucleotides	de
dc.contributor.referee	Ducho, Christian Prof. Dr.
dc.date.examination	2014-05-09
dc.description.abstracteng	It has been proposed that biopolymers need to be uniformly charged to form self-sustaining chemical systems. We have therefore established a method for the site-selective replacement of natural phosphodiester linkages of oligonucleotides (ON) by the positively charged NAA (nucleosyl amino acid) motif. The structure of these building blocks was inspired by naturally occurring muraymycin antibiotics. The phosphoramidite reagents were used to prepare various modified DNA-ON by solid phase-supported synthesis. The resultant modified ON were investigated with respect to structural and thermodynamic properties. Melting experiments revealed only a slight decrease in thermal stability of duplices bearing one NAA-modified strand. Further results indicated that mismatch recognition is not harmed and no structural distortions of duplices occur. These results challenge the hypothesis of the uniform charge pattern to be physico-chemically necessary for replicable biopolymers.	de
dc.contributor.coReferee	Diederichsen, Ulf Prof. Dr.
dc.subject.ger	Modifizierte Nucleinsäuren	de
dc.subject.ger	Nucleoside	de
dc.subject.ger	Nucleosylaminosäuren	de
dc.subject.eng	modified nucleic acids	de
dc.subject.eng	nucleosides	de
dc.subject.eng	nucleosyl amino acids	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5EB1-0-9
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	785581766

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GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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