| dc.contributor.advisor | Hell, Stefan Prof. Dr. | |
| dc.contributor.author | Machado Andrade, Débora | |
| dc.date.accessioned | 2014-06-04T08:08:51Z | |
| dc.date.available | 2014-06-04T08:08:51Z | |
| dc.date.issued | 2014-06-04 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0022-5ED7-B | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4527 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
| dc.subject.ddc | 571.4 | de |
| dc.title | On the mechanisms governing plasma membrane organization - a STED-FCS investigation | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Enderlein, Jörg Prof. Dr. | |
| dc.date.examination | 2014-01-06 | |
| dc.description.abstracteng | The plasma membrane is the barrier which localizes and separates life's
processes from their external environment. Long described as a free standing
two-dimensional
fluid mosaic, the organization of the plasma membrane
remains elusive. Currently, the most prominent models proposed for its
organization lack a consistent body of evidence for their support. Here,
we present the first validation of one of these hypotheses: the picket fence
model. Using the STED-FCS technique, we demonstrate that cortical actin
networks compartmentalize the diffusion of lipids in the plasma membrane
of cells. Interestingly, our results further indicate that the Arp2/3 complex
is a major regulator of the cortical actin networks, since inhibiting Arp2/3
leads to specific suppression of compartmentalized diffusion of lipids. Furthermore,
the extrusion of the actin cortex promoted by the introduction
of myelin basic protein networks into cells causes lipid diffusion to be modulated
by these networks instead. Conclusively, we have elucidated one of
the pivotal mechanisms governing the "fluidity" of the plasma membrane
mosaic. | de |
| dc.contributor.coReferee | Neher, Erwin Prof. Dr. | |
| dc.subject.eng | STED-FCS | de |
| dc.subject.eng | plasma membrane organization | de |
| dc.subject.eng | lipid diffusion | de |
| dc.subject.eng | cortical actin cytoskeleton | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5ED7-B-5 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.identifier.ppn | 788243500 | |