| dc.contributor.advisor | Bucher, Gregor Prof. Dr. | |
| dc.contributor.author | Oberhofer, Georg | |
| dc.date.accessioned | 2014-08-06T09:22:36Z | |
| dc.date.available | 2014-08-06T09:22:36Z | |
| dc.date.issued | 2014-08-06 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0022-5F45-D | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4640 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-4640 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
| dc.subject.ddc | 572 | de |
| dc.title | A functional analysis of a signaling center of the insect head | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Wodarz, Andreas Prof. Dr. | |
| dc.date.examination | 2014-03-07 | |
| dc.description.abstracteng | Most of our knowledge on embryonic development comes from the
fruit
y Drosophila melanogaster. An elaborate genetic model explains
the segmentation process in the trunk. However, the anterior
head region is patterned in a di erent way, and many details are not
well understood so far. This work analyzes the genetic regulations
that govern anterior head patterning in the the red
our beetle Tri-
bolium castaneum.
Especially, functional analysis with RNAi against crucial components
of the Wnt and hedgehog signaling pathways is used to reveal the
genetic interactions of these. In addition, the combination of reverse
genetics with next generation sequencing is applied to identify downstream
genetic components of these signaling pathways and to discriminate
between anterior and posterior targets in the early germ
band.
With this work I propose a new model for the patterning of the antennal
segment in Tribolium based on the cross regulation of head-gap
genes, gap-genes and segment polarity genes. The RNAseq approach
successfully identi ed target genes of the Wnt and hedgehog pathways,
as con rmed via in situ hybridization, showing the great potential
of this method. Finally, this study reveals an unexpected essential
role of Tc-senseless in hindgut establishment. | de |
| dc.contributor.coReferee | Johnsen, Steven Prof. Dr. | |
| dc.subject.eng | Wnt | de |
| dc.subject.eng | hedgehog | de |
| dc.subject.eng | RNAi | de |
| dc.subject.eng | RNAseq | de |
| dc.subject.eng | head | de |
| dc.subject.eng | growth zone | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F45-D-7 | |
| dc.affiliation.institute | Göttinger Zentrum für molekulare Biowissenschaften (GZMB) | de |
| dc.subject.gokfull | Molekularbiologie, Gentechnologie (PPN619462973) | de |
| dc.identifier.ppn | 796307970 | |