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dc.contributor.advisor Bucher, Gregor Prof. Dr.
dc.contributor.author Oberhofer, Georg
dc.date.accessioned 2014-08-06T09:22:36Z
dc.date.available 2014-08-06T09:22:36Z
dc.date.issued 2014-08-06
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-0022-5F45-D
dc.language.iso eng de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc 572 de
dc.title A functional analysis of a signaling center of the insect head de
dc.type doctoralThesis de
dc.contributor.referee Wodarz, Andreas Prof. Dr.
dc.date.examination 2014-03-07
dc.description.abstracteng Most of our knowledge on embryonic development comes from the fruit y Drosophila melanogaster. An elaborate genetic model explains the segmentation process in the trunk. However, the anterior head region is patterned in a di erent way, and many details are not well understood so far. This work analyzes the genetic regulations that govern anterior head patterning in the the red our beetle Tri- bolium castaneum. Especially, functional analysis with RNAi against crucial components of the Wnt and hedgehog signaling pathways is used to reveal the genetic interactions of these. In addition, the combination of reverse genetics with next generation sequencing is applied to identify downstream genetic components of these signaling pathways and to discriminate between anterior and posterior targets in the early germ band. With this work I propose a new model for the patterning of the antennal segment in Tribolium based on the cross regulation of head-gap genes, gap-genes and segment polarity genes. The RNAseq approach successfully identi ed target genes of the Wnt and hedgehog pathways, as con rmed via in situ hybridization, showing the great potential of this method. Finally, this study reveals an unexpected essential role of Tc-senseless in hindgut establishment. de
dc.contributor.coReferee Johnsen, Steven A. Prof. Dr.
dc.subject.eng Wnt de
dc.subject.eng hedgehog de
dc.subject.eng RNAi de
dc.subject.eng RNAseq de
dc.subject.eng head de
dc.subject.eng growth zone de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F45-D-7
dc.affiliation.institute Göttinger Zentrum für molekulare Biowissenschaften (GZMB) de
dc.subject.gokfull Molekularbiologie, Gentechnologie (PPN619462973) de
dc.identifier.ppn 796307970

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