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Transkriptionelle Regulation des pflanzlichen Detoxifikationsprogramms durch das GRAS-Protein SCL14

dc.contributor.advisorGatz, Christiane Prof. Dr.
dc.contributor.authorMeier, Alexander
dc.titleTranskriptionelle Regulation des pflanzlichen Detoxifikationsprogramms durch das GRAS-Protein SCL14de
dc.title.translatedTranscriptional regulation of the plant detoxification program by the GRAS-protein SCL14de
dc.contributor.refereeGatz, Christiane Prof. Dr.
dc.description.abstractengIn plants, the contact with toxic substances activates a group of genes, which are involved in the detoxification of these substances. A subgroup of these genes is regulated by the transcriptional coactivator Scarecrow-like 14 (SCL14), which belongs to the family of GRAS-transcriptionfactors. SCL14 interacts with the redundant bZIP transcriptionfactors TGA2, TGA5 and TGA6. The SCL14/TGA complex binds as-1-like elements in the promoters of its target genes. In this work could be shown by confocal microscopy experiments that the intracellular localization of SCL14 is altered by the phytohormon salicylic acid (SA), the xenobiotic 2,3,5-tiiodo benzoic acid (TIBA) and the oxilipin 12-oxo-phytodienoic acid (OPDA). In the untreated root, SCL14 is evenly distributed in the cytosol and the nucleus, after treatment with the mentioned substances, the protein accumulates in the nucleus. Since SA, TIBA and OPDA are electrophilic substances, the stimulus which leads to the nuclear accumulation of SCL14, could be oxidative stress. Since the Mutation of two cysteines to serines within the GRAS domain of SCL14 causes a complete loss of activity of the protein, these cysteines could be the targets of redox modifications. Transcriptional analyses of untreated and SA treated roots of wildtype and scl14/33 plants showed, that SCL14 influences specifically the SA induction but not the basal expression of some genes. For this function, the nuclear accumulation of the protein seems to play an important role. For a second group of genes, SCL14 acts as a constitutive enhancer for the basal expression as well as for the SA induced expression. In a yeast-two-hybrid experiment, NAC017 was identified as a new interaction partner of SCL14. However, the expression of the SCL14 target genes in the nac017 knockout mutant was similar to the expression in wildtype plants after treatment with SA. The interaction of SCL14 and NAC017 either does not play a role for the expression of the analysed genes or another NAC transcriptionfactor is able to take over the function of NAC017 in the
dc.contributor.coRefereeLipka, Volker Prof. Dr.
dc.subject.engGRAS proteinsde
dc.subject.engArabidopsis thalianade
dc.subject.engTGA transcription factorsde
dc.subject.engelectrophilic stressde
dc.affiliation.instituteBiologische Fakultät für Biologie und Psychologiede
dc.subject.gokfullBiologie (PPN619462639)de

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