Analyses of the Life Cycle of TLC1 and the Nuclear RNA Quality Control System in Saccharomyces cerevisiae

Wu, Haijia

dc.contributor.advisor	Krebber, Heike Prof. Dr.
dc.contributor.author	Wu, Haijia
dc.date.accessioned	2015-04-24T09:29:31Z
dc.date.available	2015-04-24T09:29:31Z
dc.date.issued	2015-04-24
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5FC3-F
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5037
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5037
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	570	de
dc.title	Analyses of the Life Cycle of TLC1 and the Nuclear RNA Quality Control System in Saccharomyces cerevisiae	de
dc.type	doctoralThesis	de
dc.contributor.referee	Krebber, Heike Prof. Dr.
dc.date.examination	2015-04-23
dc.description.abstracteng	During genome replication, chromosomes undergo a progressive shortening of their ends. To counteract this loss, a complex named telomerase functions as a reverse transcriptase to elongate telomeres. In Saccharomyces cerevisiae, telomerase contains a non-coding RNA, TLC1, which serves as a scaffold for formation of the telomerase complex and the template for the reverse transcription to elongate the telomeres. Upon its synthesis, TLC1 undergoes a series of nuclear and cytoplasmic maturation steps. Here it has been shown that the TLC1 nuclear export is dependent on the classic mRNA export pathway in addition to the already known Crm1/Xpo1 pathway. The nuclear export defects that occur upon mutation of these pathways impair the formation of the telomerase, as well as its final localisation suggesting an essential role of TLC1 shuttling in telomerase assembly. Consequently, the TLC1 nuclear transport defect leads to telomeric shortening indicating a necessity of the TLC1 shuttling for telomere maintenance. Moreover, the nuclear RNA quality control system, composed of the TRAMP complex and nuclear exosome, might mediate the nuclear maturation of TLC1. Finally, it has also been pointed out that the localisation and maturation of the nuclear quality control system might be regulated by a nuclear importer, Mtr10, which is also involved in TLC1 nuclear import.	de
dc.contributor.coReferee	Kramer, Wilfried PD Dr.
dc.contributor.thirdReferee	Braus, Gerhard Prof. Dr.
dc.contributor.thirdReferee	Ficner, Ralf Prof. Dr.
dc.contributor.thirdReferee	Pöggeler, Stefanie Prof. Dr.
dc.contributor.thirdReferee	Heimel, Kai Prof. Dr.
dc.subject.eng	TLC1	de
dc.subject.eng	non-coding RNA	de
dc.subject.eng	Telomerase	de
dc.subject.eng	nuclear transport	de
dc.subject.eng	Mex67	de
dc.subject.eng	Xpo1	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5FC3-F-9
dc.affiliation.institute	Biologische Fakultät für Biologie und Psychologie	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	823311317

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