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dc.contributor.advisor Krebber, Heike Prof. Dr.
dc.contributor.author Wu, Haijia
dc.date.accessioned 2015-04-24T09:29:31Z
dc.date.available 2015-04-24T09:29:31Z
dc.date.issued 2015-04-24
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-0022-5FC3-F
dc.language.iso eng de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc 570 de
dc.title Analyses of the Life Cycle of TLC1 and the Nuclear RNA Quality Control System in Saccharomyces cerevisiae de
dc.type doctoralThesis de
dc.contributor.referee Krebber, Heike Prof. Dr.
dc.date.examination 2015-04-23
dc.description.abstracteng During genome replication, chromosomes undergo a progressive shortening of their ends. To counteract this loss, a complex named telomerase functions as a reverse transcriptase to elongate telomeres. In Saccharomyces cerevisiae, telomerase contains a non-coding RNA, TLC1, which serves as a scaffold for formation of the telomerase complex and the template for the reverse transcription to elongate the telomeres. Upon its synthesis, TLC1 undergoes a series of nuclear and cytoplasmic maturation steps. Here it has been shown that the TLC1 nuclear export is dependent on the classic mRNA export pathway in addition to the already known Crm1/Xpo1 pathway. The nuclear export defects that occur upon mutation of these pathways impair the formation of the telomerase, as well as its final localisation suggesting an essential role of TLC1 shuttling in telomerase assembly. Consequently, the TLC1 nuclear transport defect leads to telomeric shortening indicating a necessity of the TLC1 shuttling for telomere maintenance. Moreover, the nuclear RNA quality control system, composed of the TRAMP complex and nuclear exosome, might mediate the nuclear maturation of TLC1. Finally, it has also been pointed out that the localisation and maturation of the nuclear quality control system might be regulated by a nuclear importer, Mtr10, which is also involved in TLC1 nuclear import. de
dc.contributor.coReferee Kramer, Wilfried PD Dr.
dc.contributor.thirdReferee Braus, Gerhard Prof. Dr.
dc.contributor.thirdReferee Ficner, Ralf Prof. Dr.
dc.contributor.thirdReferee Pöggeler, Stefanie Prof. Dr.
dc.contributor.thirdReferee Heimel, Kai Prof. Dr.
dc.subject.eng TLC1 de
dc.subject.eng non-coding RNA de
dc.subject.eng Telomerase de
dc.subject.eng nuclear transport de
dc.subject.eng Mex67 de
dc.subject.eng Xpo1 de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5FC3-F-9
dc.affiliation.institute Biologische Fakultät für Biologie und Psychologie de
dc.subject.gokfull Biologie (PPN619462639) de
dc.identifier.ppn 823311317

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