| dc.contributor.advisor | Meijere, Armin De Prof. Dr. | |
| dc.contributor.author | Sednev, Maksim | |
| dc.date.accessioned | 2015-07-02T11:16:47Z | |
| dc.date.available | 2015-07-02T11:16:47Z | |
| dc.date.issued | 2015-07-02 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0022-6041-F | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-5165 | |
| dc.language.iso | eng | de |
| dc.publisher | Niedersächsische Staats- und Universitätsbibliothek Göttingen | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 540 | de |
| dc.title | Fluorescent Dyes with Large Stokes Shifts of 80−200 nm for Optical Microscopy and Nanoscopy | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Meijere, Armin De Prof. Dr. | |
| dc.date.examination | 2015-06-08 | |
| dc.description.abstracteng | Bright and photostable fluorescent dyes with large Stokes shift are rare, though they are indispensable in optical microscopy, biology and chemistry. The rapid progress in super-resolution microscopy based on stimulated emission depletion (STED) phenomenon encouraged us to design and prepare new coumarins and a hybrid carborhodol dye. Variation of electron-withdrawing groups at C-3 and/or C-4 enabled us to create promising coumarin dyes possessing a 3-(2-pyridyl) group (λabs/λem = 432/512 nm in aqueous phos-phate buffer), a 3-(pyrido[1,2-a]pyrrolo[2,1-c]pyrazinium) group (489/587 nm in MeOH) and a fused quinoline ring (453/617 nm in aqueous phosphate buffer). The new dyes were decorated with a polar phosphate group which provided sufficient solubility in aqueous solutions and a carboxylic group which was required for bioconjugation.
The hybrid carborhodol dye was obtained by a combination of (carbo)fluorescein and carbopyronine fluorophores. Due to the broad absorption and emission spectra of the carborhodol in the conjugated form (586/613 nm in aqueous phosphate buffer), the effective Stokes shift is larger in comparison with small Stokes shifts of the parent dyes. This allowed the use of carborhodol in two-color imaging schemes as well as in STED micros-copy with a 775 nm depletion laser. | de |
| dc.contributor.coReferee | Hell, Stefan Prof. Dr. | |
| dc.subject.eng | fluorescent dyes | de |
| dc.subject.eng | super-resolution microscopy | de |
| dc.subject.eng | STED | de |
| dc.subject.eng | organic synthesis | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0022-6041-F-2 | |
| dc.affiliation.institute | Fakultät für Chemie | de |
| dc.subject.gokfull | Chemie (PPN62138352X) | de |
| dc.identifier.ppn | 828805989 | |