Synthesis of fluorescent toxin and nucleotide derivatives to specifically address membrane proteins

by Hanna Agnes Radzey
Doctoral thesis
Date of Examination:2015-04-01
Date of issue:2015-07-15
Advisor:Prof. Dr. Ulf Diederichsen
Referee:Prof. Dr. Ulf Diederichsen
Referee:Prof. Dr. Andreas Janshoff
Referee:Prof. Dr. Lutz Ackermann
Referee:Prof. Dr. Konrad Koszinowski
Referee:Prof. Dr. Claudia Höbartner
Referee:Dr. Inke Siewert
Persistent Address: http://dx.doi.org/10.53846/goediss-5177

 

 

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Abstract

English

Within this project, new tools for in vivo/in vitro labelling were developed and investigated, which may be utilized in the future to elucidate structures, localisation and distinct processes of biomolecules in their native environment. Fluorescence labelling and detection provides researchers with a highly sensitive, non-destructive method to visualize biomolecules even on the nanometre scale. This thesis comprises two different approaches for fluorescent labelling. The first approach includes the indirect labelling of ion channels, in which ion channels are addressed by specific binding fluorescently labelled neurotoxins. The second project involves a direct labelling approach, where a nucleotide is functionalized with a fluorophore for direct investigation of its concentration and localisation.
Keywords: fluorescent labelling; conotoxin; pompilidotoxin; iberiotoxin; cAMP; disulphide rich peptides; caging
 
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