Synthesis and Investigation of Nucleobase Functionalized β-Peptide as SNAREs Model System for Membranefusion

Sadek, Muheeb

dc.contributor.advisor	Diederichsen, Ulf Prof. Dr.
dc.contributor.author	Sadek, Muheeb
dc.date.accessioned	2015-07-22T09:39:41Z
dc.date.available	2015-07-22T09:39:41Z
dc.date.issued	2015-07-22
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-605D-6
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5194
dc.language.iso	eng	de
dc.publisher	Niedersächsische Staats- und Universitätsbibliothek Göttingen	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	540	de
dc.title	Synthesis and Investigation of Nucleobase Functionalized β-Peptide as SNAREs Model System for Membranefusion	de
dc.type	doctoralThesis	de
dc.contributor.referee	Steinem, Claudia Prof. Dr.
dc.date.examination	2015-05-26
dc.description.abstracteng	In this work, the novel simplified SNARE-mimicking systems consisting of TMD/linker segments of the natural membrane-bound SNARE proteins (syntaxin-1A and synaptobrevin 2) with a nucleobase functionalized β-peptide (β-PNA) recognition motif were designed and synthesized. The β-PNAs allow antiparallel orientation and therefore, a fusion mechanism is addressed, that differs from the native SNARE situation. Nevertheless, the artificial TMD/linker/β-PNA constructs are surprisingly efficient in mediating membrane fusion. The β-PNA recognition motif with the linear and conformational rigid 14-helix backbone offers an especially interesting option of distance control between the vesicles. This system offers a powerful tool to control the fusion state by using different length of β-PNA. The extended β-PNA leads to hemifusion state while the shortened one induces full fusion.	de
dc.contributor.coReferee	Jahn, Reinhard Prof. Dr.
dc.subject.eng	SNARE protein	de
dc.subject.eng	Fusion distance controlling	de
dc.subject.eng	Parallel fusion system	de
dc.subject.eng	Nucleobase functionalized β-peptide	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-605D-6-2
dc.affiliation.institute	Fakultät für Chemie	de
dc.subject.gokfull	Chemie (PPN62138352X)	de
dc.identifier.ppn	831913037

Dateien

Name:Dissertation Sadek-2015.pdf

Größe:3.969Mb

Format:PDF

Öffnen

Name:: Dissertation Sadek-2015.pdf
Größe:: 3.969Mb
Format:: PDF

Öffnen

Das Dokument erscheint in:

Fakultät für Chemie (inkl. GAUSS) [701]

Zur Kurzanzeige