DNA-based molecular force sensors in cytoskeletal networks and cells

by Meenakshi Prabhune
Doctoral thesis
Date of Examination:2015-07-10
Date of issue:2015-07-30
Advisor:Dr. Florian Rehfeldt
Referee:Prof. Dr. Christoph F. Schmidt
Referee:Prof. Dr. Sarah Köster
Persistent Address: http://dx.doi.org/10.53846/goediss-5206

 

 

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Abstract

English

Eukaryotic cells are highly active and undergo force generating processes such as cell adhesion, migration and division. The cytoskeleton, a polymer meshwork in cells, plays an important role in these cellular processes. Advances in the field of biomechanics have now made it possible to measure the cellular forces in the pN range. We have designed a DNA force sensor, based on FRET, as a tool to investigate multiple cellular processes. The following work encompasses design of the DNA force sensor and validation of its working through control experiments. The sensor was tested in vitro in cytoskeletal networks; mechanics of which were characterized using macrorheology. Preliminary in vivo experiments wherein the DNA force sensor was introduced inside cells are also described.
Keywords: DNA; force sensor; FRET; rheology; networks; mechanics
 
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