• Deutsch
    • English
  • English 
    • Deutsch
    • English
  • Login
Item View 
  •   Home
  • Medizin
  • Human- und Zahnmedizin
  • Item View
  •   Home
  • Medizin
  • Human- und Zahnmedizin
  • Item View
JavaScript is disabled for your browser. Some features of this site may not work without it.

miR-125 regulates niche organization in Drosophila melanogaster ovary by affecting Notch signaling pathway via its target Tom

by Berenike Johanna Bögeholz
Doctoral thesis
Date of Examination:2015-08-19
Date of issue:2015-08-11
Advisor:Prof. Dr. Matthias Dobbelstein
Referee:Prof. Dr. Tomas Pieler
Referee:Prof. Dr. Margarete Schön
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-5215

 

 

Files in this item

Name:MDThesis_SUBonlineVersion .pdf
Size:1.13Mb
Format:PDF
ViewOpen

The following license files are associated with this item:


Abstract

English

For the maintenance of tissue homeostasis upon injury or natural cell turnover, a constant source of new cells is needed. Usually these cells are delivered by tissue specific adult stem cells. The stem cells reside in a specialized microenvironment, called the niche. This niche provides an environment controlling adult stem cell proliferation and maintenance. Defects in the formation of the niche may result in stem cell loss or overproliferation, and can lead to a l ack of tissue regeneration or cancer formation. A better understanding of stem cells in their natural environment may help to understand the development of certain diseases, such as cancer, and may change their treatment. Previously, microRNAs were shown to participate in stem cell regulation in vivo. This study investigated the role of microRNA miR-125 (part of the conserved let-7-Complex) in the Drosophila melanogaster germarium. The Drosophila germarium hosts germline stem cells (GSCs) and is an ideal model system to study the interaction between stem cells and their niche. In this study, we show that miR-125 participates in the establishment of proper GSC niche. Strikingly, the GSC niche was expanded upon loss or overexpression of miR-125 and hosted an increased number of stem cells. This developmental niche expansion upon aberrant miR-125 levels shows similarities to the proposed expansion of ‘precancer niches’ promoting the formation and progression of mammalian tumors. We have found that miR-125 targets Tom, a member of the Bearded family, involved in the regulation of the highly conserved Notch pathway. As the transcription of miR-125 is triggered by ecdysone, a steroid hormone in the fly, miR-125 can be proposed as a linkage between steroid hormones and the prominent Notch pathway. Thus, miR-125 may help to regulate Notch signaling spatially and temporally.
Keywords: miRNA; Drosophila melanogaster; Notch-Pathway; miR-125; stem cell; niche
 

Statistik

Publish here

Browse

All of eDissFaculties & ProgramsIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesTypeThis FacultyIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesType

Help & Info

Publishing on eDissPDF GuideTerms of ContractFAQ

Contact Us | Impressum | Cookie Consents | Data Protection Information
eDiss Office - SUB Göttingen (Central Library)
Platz der Göttinger Sieben 1
Mo - Fr 10:00 – 12:00 h


Tel.: +49 (0)551 39-27809 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
ediss_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]
Göttingen State and University Library | Göttingen University
Medicine Library (Doctoral candidates of medicine only)
Robert-Koch-Str. 40
Mon – Fri 8:00 – 24:00 h
Sat - Sun 8:00 – 22:00 h
Holidays 10:00 – 20:00 h
Tel.: +49 551 39-8395 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
bbmed_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]