| dc.contributor.advisor | Schmitt, Andrea Prof. Dr. | |
| dc.contributor.author | Shariati, Jawid | |
| dc.date.accessioned | 2017-05-18T08:17:35Z | |
| dc.date.available | 2017-06-08T22:50:07Z | |
| dc.date.issued | 2017-05-18 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0023-3E4D-5 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6301 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6301 | |
| dc.language.iso | deu | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 610 | de |
| dc.title | Immunhistochemische Untersuchung von Oligodendrozyten im post- mortem Hippokampus bei Schizophrenie | de |
| dc.type | doctoralThesis | de |
| dc.title.translated | Immunohistochemical Analysis of Oligodendrocytes in post - mortem Hippocampus in Schizophrenia | de |
| dc.contributor.referee | Schmitt, Andrea Prof. Dr. | |
| dc.date.examination | 2017-06-01 | |
| dc.description.abstracteng | The prevalence of schizophrenia is about 1%, and the disease is also one of the most common causes of life-threatening diseases due to the age of the disease in young adulthood and frequent social disability. In the pathogenesis of the disease, a neuronal developmental disorder has been postulated during the perinatal phase, which could contribute to the development of schizophrenia. The human hippocampus is an important center, which is involved in memory processes and, together with the amygdalae, in the regulation of affection. In patients with schizophrenia, a functional disorder of the hippocampus was postulated, so the patients have cognitive deficits and disturbances of the emotional regulation. Studies using structural magnetic resonance imaging (sMRT) and post-mortem studies have shown volume loss in the medial temporal region especially the hippocampus, in schizophrenia. In particular, magnetic Transfer Imaging (MTI) and diffusion Tensor Imaging (DTI) results showed a reduction of Myelin and/or axonal membranes, and disorders of the fractional anisotropy of the white matter as a sign of disconnectivity especially in the fornix of the hippocampus. Recent studies using design-based Stereology and study of neurons, Oligodendrocytes, and astroglial cells in different subregions of the posterior part of the hippocampus showed a reduction in the number and density of Oligodendrocytes in the right and left Subregion of the hippocampal CA4. In contrast, the number of neurons proved to be unchanged. The results of a reduced oligodendrocyte count suggest a disturbed connectivity of CA4 in the posterior part of the hippocampus as a possible pathological mechanism. The significantly reduced number of oligodendrocytes was also detected in post-mortem stereological studies using Nissl- staining.
Therefore, in the present study immunohistochemical staining was used to study ripe oligodendrocyte populations in the posterior hippocampus of schizophrenic patients and healthy controls. Furthermore, by immunohistochemical-based quantification, the more investigator-dependent quantification of Nissl-stained cells should be verified. In this respect, we examined tissue sections of 10 patients and 10 controls. The density of two oligodendrocyte populations in CA1, 2/3, 4, and the Subiculum of the posterior part of the hippocampus were determined. The investigation was carried out by means of specific immunohistochemical staining with OLIG1 and OLIG2 antibodies. Thus, OLIG1 detected the more immature and OLIG2 the more mature myelinated oligodendrocytes in subregions of the hippocampus.
In our work we found a trend towards reduced cell density in CA2 / 3 on the right as well as in CA4 on OLIG1 positive oligodendrocytes. There was no significant difference between the patient and the control group for the OLIG2-immunopositive cells. This is a proof that only a subpopulation of the oligodendrocytes with a lower maturity level in schizophrenia is affected. However, duo to a variety of statistical tests and without Bonferroni correction for multiple tests, the finding could be considered false positive. According to our results there is therefore no reduction in the immunohistochemically measured density of oligodendrocytes in schizophrenia in the posterior part of hippocampus. However, in contrast to stereological investigations of a whole subregion, dense measurements are associated with methodological errors and are possibly influenced by different shrinkage of the tissue, for example by disease influence, fixation and staining methods. | de |
| dc.contributor.coReferee | Stadelmann-Nessler, Christine Prof. Dr. | |
| dc.contributor.thirdReferee | Reuss, Bernhard Prof. Dr. | |
| dc.subject.ger | Schizophrenie, Oligodendrozyten, Hippokampus, Immunhistochemie | de |
| dc.subject.eng | Schizophrenia, Oligodendrocytes, Hippocampus, Immunhistochemistry, | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0023-3E4D-5-1 | |
| dc.affiliation.institute | Medizinische Fakultät | de |
| dc.subject.gokfull | Psychiatrie (PPN619876344) | de |
| dc.description.embargoed | 2017-06-08 | |
| dc.identifier.ppn | 887726046 | |