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Fluorescence correlation spectroscopy for studying intermediate filament assembly

dc.contributor.advisorKöster, Sarah Prof. Dr.
dc.contributor.authorSchroeder, Viktor
dc.date.accessioned2017-08-10T09:35:10Z
dc.date.available2017-08-10T09:35:10Z
dc.date.issued2017-08-10
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-0023-3ED1-8
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-6436
dc.language.isoengde
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc571.4de
dc.titleFluorescence correlation spectroscopy for studying intermediate filament assemblyde
dc.typedoctoralThesisde
dc.contributor.refereeKöster, Sarah Prof. Dr.
dc.date.examination2017-08-04
dc.description.abstractengIntermediate filaments play a central role in the cytoskeleton of eukaryotic cells. Together with microtubules and actin filaments they determine the mechanical properties of cells. Microtubules are also the guiding tracks for molecular transport in cells while actin filaments play an essential role in cell motility. The diameter of intermediate filaments lies between the diameter of actin filaments and microtubules which led to their name. There is an enormous genetic variety of intermediate filaments. In humans there are over 70 different genes for intermediate filament proteins. The different types of intermediate filaments are cell type-specific. For example, the intermediate filament protein vimentin is found in cells of mesenchymal origin and keratins occur in epithelial cells. Mutations in genes coding for intermediate filaments are known to cause more than 80 diseases, among them Alexander disease and amyotrophic lateral sclerosis (ALS). Intermediate filaments share a common hierarchical assembly scheme. Several tetramers form unit length filaments (ULFs). The ULFs then anneal longitudinally to form elongated filaments. Studies of the assembly are necessary to understand the structural properties of cells. In this thesis we aim to further understand the assembly of intermediate filaments. We use fluorescence correlation spectroscopy (FCS) which is a versatile technique to study the motility of molecules in solution. During the thesis project, we built a setup suitable for FCS. It allows for detecting the diffusive properties of the different states during the assembly. With the setup we study both the early and the long time scales of the assembly of vimentin filaments. For the long times we employ bulk measurements. To access the early time scales of the assembly, we employ microfluidic techniques. With the microfluidic mixing device we map the temporal axis to a spatial axis. This gives us the possibility to control the interaction of the molecules in a defined manner. We aim to observe the assembly reaction of vimentin intermediate filaments upon the addition of monovalent salt ions.de
dc.contributor.coRefereeRehfeldt, Florian Dr.
dc.subject.engFluorescence correlation spectroscopyde
dc.subject.engFCSde
dc.subject.engintermediate filamentsde
dc.subject.engIFde
dc.subject.engvimentinde
dc.subject.engmicrofluidicsde
dc.subject.engcytoskeletonde
dc.identifier.urnurn:nbn:de:gbv:7-11858/00-1735-0000-0023-3ED1-8-2
dc.affiliation.instituteGöttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)de
dc.subject.gokfullBiologie (PPN619462639)de
dc.identifier.ppn895450801


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