Untersuchung der Transportvorgänge des Prolyl-Hydroxylase-Hemmers ICA an den Transportern OAT1, OAT2, OAT3 und OAT4 von proximalen Nierentubuluszellen

Schulz, Kei

dc.contributor.advisor	Burckhardt, Birgitta-Christina Prof. Dr.
dc.contributor.author	Schulz, Kei
dc.date.accessioned	2017-10-27T07:16:14Z
dc.date.available	2017-12-12T23:50:05Z
dc.date.issued	2017-10-27
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0023-3F4A-3
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6550
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Untersuchung der Transportvorgänge des Prolyl-Hydroxylase-Hemmers ICA an den Transportern OAT1, OAT2, OAT3 und OAT4 von proximalen Nierentubuluszellen	de
dc.type	doctoralThesis	de
dc.title.translated	Examination of transport processes of the prolyl hydroxylase inhibitor ICA on the transporters OAT1, OAT2, OAT3 and OAT4 of renal proximal tubule cells	de
dc.contributor.referee	Burckhardt, Birgitta-Christina Prof. Dr.
dc.date.examination	2017-12-05
dc.description.abstracteng	This thesis discusses the interactions between ICA and the organic anion transporters OAT1, OAT2, OAT3 and OAT4. ICA is a substance which can inhibit the prolyl hydroxylase domain. This leads to an upregulation of hypoxia inducible factor (HIF). Artificial upregulation of HIF has got the opportunity to protect the organism from hypoxia related diseases and effects. Renal ischemia plays an important part in the pathogenesis of acute and chronic kidney failure. To measure the impact of ICA on the different organic anion transporters uptake experiments with these transporters stably transfected in human embryonic kidney 293 cells and its reference substrates were carried out. The first question was whether there is any interaction or inhibition of the organic anion transporters. To quantify the inhibition the half maximal inhibitory concentration was measured for each OAT. In the last step it was examined whether ICA is transported by the organic anion transporters. The results show that OAT1, OAT3 and OAT4 are inhibited by ICA whereas OAT2 only marginally interacts with ICA. Furthermore, ICA is transported by OAT3 and OAT4. In conclusion the results of this thesis strengthen the hypothesis that ICA can be transported by the organic anion transporters into the proximal tubule cells to upregulate HIF and prevent hypoxia induced kidney failure.	de
dc.contributor.coReferee	Gross, Oliver Prof. Dr.
dc.subject.eng	organic anion transporter	de
dc.subject.eng	OAT	de
dc.subject.eng	PHD inhibitor	de
dc.subject.eng	ICA	de
dc.subject.eng	hypoxia	de
dc.subject.eng	HEK293	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0023-3F4A-3-8
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.description.embargoed	2017-12-12
dc.identifier.ppn	1002331072

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