dc.contributor.advisor | Jackson, Daniel John Prof. Dr. | |
dc.contributor.author | Germer, Juliane | |
dc.date.accessioned | 2017-12-05T09:55:17Z | |
dc.date.available | 2017-12-05T09:55:17Z | |
dc.date.issued | 2017-12-05 | |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0023-3F9B-B | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6604 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6604 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6604 | |
dc.language.iso | eng | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.ddc | 910 | de |
dc.subject.ddc | 550 | de |
dc.title | Insights into the Holobiont of the Early Branching Metazoan Vaceletia sp. and its Biomineralization Strategy | de |
dc.type | doctoralThesis | de |
dc.contributor.referee | Jackson, Daniel John Prof. Dr. | |
dc.date.examination | 2017-09-13 | |
dc.description.abstracteng | Sponges are evolutionary and ecologically very successful animals. They are abundant and
significant members of benthic communities throughout the world’s oceans. Having
emerged during the Neoproterozoic era, sponges are among the most ancient of all extant
animal lineages. Due to this basal branching position sponges are ideal models to gain
insights into the origin and evolution of important metazoan traits. This thesis addresses
two important aspects of sponge biology. The first part focuses on host-microbe
interactions of the hypercalcifying demosponge Vaceletia sp. Like Vaceletia, most sponges
comprise dense and diverse microbial communities that can constitute up to 50% of the
sponge’s biomass. The analysis and characterization of Vaceletia sp. holo-transcriptome,
and the comparison to other sponge transcriptomes and genomes shows that this sponge
interacts in various ways with its microbial community. The results of my study imply that
the sponge immune system as well as eukaryotic–like proteins from bacteria play an
important role in mediating interactions. By studying the underlying molecular
mechanisms of lipid pathway components, I showed that short chain fatty acids and midchain branched fatty acids are most likely produced by the sponge’s bacterial community,
whereas long chain fatty acids are most likely synthesized by the sponge itself via
elongation and desaturation of short-chain precursors. The second part of this thesis
focuses on the biomineralization strategy employed by Vaceletia sp. By generating a
comprehensive skeletal proteome of this sponge, I identified and characterized 40 proteins
that most likely represent the majority of components playing an important role in the
mineralization process of Vaceletia. The proteome contains components showing
similarities to already identified proteins with a known role in biomineralization as well as
novel components. The microbial community of Vaceletia sp. apparently plays a minimal
role in directly contributing proteinaceous compounds to the skeleton formation in this
sponge. This thesis represents the first investigation into the molecular mechanisms
underlying sponge-microbe interactions and the biomineralization of the early branching
Vaceletia sp. The studied interactions include innate immunity, eukaryotic-like proteins in
bacteria and metabolic interactions. The results of my thesis expand our knowledge of the
complex gene repertoire of sponges, show the importance of metabolic interactions
between Vaceletia and its microbial community, and give insight into the
biomineralization strategy of this sponge. | de |
dc.contributor.coReferee | Reitner, Joachim Prof. Dr. | |
dc.subject.eng | biomineralization | de |
dc.subject.eng | sponge | de |
dc.subject.eng | microbes | de |
dc.subject.eng | symbiosis | de |
dc.subject.eng | transcriptome | de |
dc.subject.eng | metabolism | de |
dc.subject.eng | fatty acids | de |
dc.subject.eng | immunity | de |
dc.subject.eng | signalling pathway | de |
dc.subject.eng | extracellular matrix | de |
dc.subject.eng | proteome | de |
dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0023-3F9B-B-2 | |
dc.affiliation.institute | Fakultät für Geowissenschaften und Geographie | de |
dc.subject.gokfull | Geologische Wissenschaften (PPN62504584X) | de |
dc.identifier.ppn | 1007232811 | |