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Optical characterization of ligand-induced staining of olfactory receptor neurons in Xenopus laevis

dc.contributor.advisorSchild, Detlev Prof. Dr. Dr.
dc.contributor.authorMgbor, Nwadiuto Amara
dc.date.accessioned2015-10-07T09:20:47Z
dc.date.available2015-10-07T09:20:47Z
dc.date.issued2015-10-07
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-0023-963E-A
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-5297
dc.language.isoengde
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc610de
dc.titleOptical characterization of ligand-induced staining of olfactory receptor neurons in Xenopus laevisde
dc.typedoctoralThesisde
dc.contributor.refereeMeyer, Alexander PD Dr.
dc.date.examination2015-09-30
dc.description.abstractengThis thesis aimed at investigating ligand-induced staining (LIS) as a new labelling technique to visualize ORN subpopulation in larval X.l. In the course of this study, it was at first validated that LIS is a suitable technique for this experimental model animal. Secondly, tests carried out with forskolin furthermore revealed that the observed staining does not occur via activated cAMP/CNG channels. To generate first evidence that an odorant-induced, receptor-mediated endocytosis could be a plausible explanation for the observed staining, a temperature experiment was conducted, knowing that a decrease in temperature affects receptor-mediated endocytosis in sensory cells of vertebrates. As a decrease in staining efficiency was observed in these experiments, in summary it can be hypothesized that, presumably based on an adaption mechanism, a receptor-mediated endocytosis is the reason for the observed ligand-induced staining.de
dc.contributor.coRefereeOppermann, Martin Prof. Dr.
dc.subject.engXenopus laevisde
dc.subject.engligand-induced endocytosisde
dc.identifier.urnurn:nbn:de:gbv:7-11858/00-1735-0000-0023-963E-A-4
dc.affiliation.instituteMedizinische Fakultätde
dc.subject.gokfullMedizin (PPN619874732)de
dc.subject.gokfullNeuroanatomie, Neurophysiologie, Neuropathologie (PPN619876255)de
dc.identifier.ppn836298985


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